中文摘要：

重建的胚胎从 intersubspecies 导出体的房间原子转移(SCNT ) 从 intrasubspecies SCNT 比那些有更差的发展潜力。把那家荷兰的一种乳牛奶店基于我们的以前的学习牛( HD )在施主 karyoplast 和接受者胞质之间的 mitochondrial DNA ( mtDNA ) haplotype 相容性为 SCNT 胚胎开发是关键的，我们根据聚合酶链反应限制决定的 mtDNA haplotypes 作为接受者胞质作为施主 karyoplast 和 Luxi 黄小母牛( LY )用 HD 执行了 intersubspecies SCNT 碎片长度多型性( PCR-RFLP )分析。结果证明 intersubspecies mtDNA homotype SCNT 胚胎象改进胚囊 reprogramming 地位一样比 intrasubspecies mtDNA heterotype 胚胎有更高的培植前和培植以后的发展胜任，包括象 Oct4 和 Sox2 那样的 pluripotent 基因的正常 H3K9 dimethylation 模式和倡导者 hypomethylation ，用 LY 卵母细胞建议那 intersubspecies SCNT 维持克隆效率的 HD 并且可以发展成正常的改编 HD 原子核克隆动物 ulti 我们的结果显示 karyoplastcytoplast 相互作用和 mtDNA haplotype 相容性可以影响牛的 intersubspecies SCNT 效率。牛的 intersubspecies SCNT 上的这研究为理解在影响牛的 SCNT 效率的 karyoplast 和胞质之间的 mtDNA haplotype 相容性的机制是珍贵的，并且为克隆的 HD 提供一个其他、经济的资源。

英文摘要：

Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer （SCNT） have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine （HD） mitochondrial DNA （mtDNA） haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer （LY） as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal cloned animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.