中文摘要：

探讨人皮肤角质形成细胞（humankeratinocytes，HKC）对成纤维细胞（humanfibroblasts．HFB）增殖及胶原合成的影响，对于临床治疗增生性瘢痕有重要的理论和实际意义。DMEM与HKC上清液的混合液（1：1）培养HFB，3．4kPa气体压力加载5h或10h；MTT法测定HFB增殖：羟脯胺酸比色法测定HFB上清液中胶原蛋白合成量。实验结果显示，静态或加压时，HKC组与K-SFM组比较，HFB数量及胶原合成量显著增多（P〈0．05）；压力组与无压力组比较．HFB数量及胶原合成量显著下降（P〈0．05）；HKC且加压10h组与K-SFM且静态10h组比较，HFB数量显著增多（P〈0．05），胶原合成量显著下降（P〈0．05）。以上结果说明，HKC上清液可以促进HFB增殖及胶原的合成，3．4kPa气体压力可以抑制HFB增殖及胶原的合成。

英文摘要：

Human fibroblasts （HFB） is responsible for treating hypertrophic scars due to their proliferation and the corresponding collagen secretion, which are influenced by human keratinocytes cells （HKC）. This influence was investigated in the present work. HFB were cultured in a mixed solution with 50% HKC supernatant and 50% DMEM. This cultivation process was performed under 3.4 kPa pressure within 5 and 10 h, respectively. In addition, the cultivation without pressure was settled as the control group. MTT method was used to determine the proliferation of HFB, while coloricmetric method was utilized to identify the collagen expression in the supernatant of HFB. The experimental results revealed that HFB proliferation and collagen were increased significantly in the supernatant of HKC groups （P〈0.05） with and without pressure. Compared to the control group, reduction of HFB proliferation and collagen significantly happened in the groups with 3.4 kPa pressure （P〈0.05）. However, in the HKC group under 3.4 kPa pressure for 10 h, HFB proliferation was improved significantly （P〈0.05） but collagen was decreased significantly （P〈0.05）. This investigation suggested that the supernatant of HKC played an important role in improving HFB proliferation and collagen, which was inhibited by 3.4 kPa pressure.