中文摘要：

目的：检测卵巢肿瘤中自噬相关基因5（Atg5）的表达情况,公共数据库分析Atg5在mRNA表达水平与患者预后的相关性。研究人卵巢癌细胞系SKOV3中Atg5下调对细胞增殖的影响。方法：收集卵巢肿瘤患者卵巢肿瘤标本共30例,对其进行Atg5的免疫组织化学染色,检测其在不同卵巢肿瘤患者中的表达情况。应用公共数据库Kaplan-Meier plots对多组卵巢癌患者mRNA表达谱分析,预测Atg5的表达水平对患者预后的影响。SKOV3细胞分为两组,阴性对照组（转染阴性对照siRNA）和实验组（转染Atg5 siRNA）。通过蛋白印记法检测、CCK-8法及克隆形成实验检测细胞转染siRNA后Atg5的表达及增殖情况。结果：免疫组织化学检测卵巢肿瘤组织Atg5表达情况,卵巢癌组阳性率（75.00%）较交界性卵巢肿瘤（40.00%）及良性卵巢肿瘤（20.00%）表达水平高,且卵巢癌组与良性卵巢肿瘤组差异有统计学意义（P〈0.05）。公共数据库KaplanMeier plots表达谱芯片分析Atg5 mRNA表达水平对患者预后的影响,结果显示高表达Atg5组较低表达组预后差,差异有统计学意义〔（P=3.1e-05,HR=1.37（1.18,1.58）〕。对人卵巢癌细胞系SKOV3进行反义siRNA干扰,干扰后细胞采用CCK8法分别在不同时间点检测细胞的增殖能力,结果阴性对照组和转染Atg5 siRNA组（实验组）A450值在24 h、48 h、72 h对照组及实验组A450值分别为1.179±0.036、1.454±0.040、1.828±0.059;0.640±0.039、1.032±0.054 1.146±0.040,实验组较阴性对照组明显降低,差异有统计学意义（均P〈0.01）。结论：免疫组织化学法检测Atg5在卵巢癌中表达与良性卵巢肿瘤及交界性卵巢肿瘤相比明显升高,对公共数据库mRNA表达谱芯片的分析显示,Atg5高表达可能与卵巢癌的不良预后有关,反义干扰siRNA显著下调了Atg5水平表达的SKOV3细胞,其增殖能力受到明显抑制。

英文摘要：

Objective： To detect the expressions of autophagy - related gene 5 in ovarian tumors, analyze the correlation between autophagy - related gene 5 mRNA expression level and prognosis based on common data base, research the effect of down - regulation of antophagy L related gcne 5 on cell proliferation of ovarian cancer SKOV3 cell line. Methods： Thirty patients with ovarian tumors were collected, immunohistoehemieal method was used to detect the expression levels of autophagy - related gene 5 in patients with different ovarian tumors; Common database Kaplan - Meier plots was used to analyze the expression levels of autophagy - related gene 5 mRNA and predict the effects of autophagy - related gene 5 expression levels on prognosis. SKOV3 cells were divided into negative control group （ transfected with negative control siRNA） and experimental group （transfeeted with autophagy -related gene 5 siRNA） . Western blotting, CCK -8 assay and colony formation assay were used to detect the expressions and proliferation of autophagy - related gene 5. Results： The positive rate of autophagy - related gene 5 in ovarian cancer group was 75.00% , which was statistically significantly higher than those in borderline ovarian tumor group （40. 00% ） and benign ovarian tumor group （20. 00% ） , there was statistically significant difference between ovarian cancer group and benign ovarian tumor group （ P 〈 0. 05 ） . The prognosis of patients with high expression of autophagy - related gene 5 was poorer than that of patients with low expression of autophagy - related gene 5 [ （ P = 3.1 e - 05, HR = 1.37 （ 1.18, 1.58 ） 3 . Antisense siRNA in- terference was performed among human ovarian cancer SKOV3 cell line, then CCK8 assay was used to detect the proliferation abilities of hu- man ovarian cancer SKOV3 cells at different time points, A450 values at 24, 48 and 72 hours in negative control group and experimental group were 1. 179 ±0. 036, 1. 454 ±0. 040, 1. 828 ± 0. 059 and 0. 640 ±0. 039, 1. 032 ±0. 0