中文摘要：

目的筛选出结肠癌细胞侵袭相关研究的最佳培养基。方法在RPMI1640、DMEM、DMEM-F12培养基中对人结直肠癌细胞株HCT-116、LOVO、CACO-2进行常规培养，采用集落形成实验检测肿瘤细胞增殖能力，免疫荧光检测上皮型钙黏素（E-cadherin）、波形蛋白（vimentin）、Nm23-H1、b53蛋白表达，RT-PCR检测Nm23-H1、p53、蜗牛基因（snail-1）mRNA表达。结果与RPMI-1640、DMEM培养组相比，DMEM-F12培养组三种人结直肠癌细胞克隆集落形成率、vimentin、p53蛋白、snail-1和p53mRNA表达均明显增加（P〈0．05），而E-cadherin、Nm23-H1蛋白表达和Nm23-H1mRNA表达降低（P〈0．05）；RPMI1640培养组与DMEM培养组间各观察指标差异均无统计学意义（P〉O．05）。结论DMEM-F12培养基相对DMEM、RPMI1640培养基更适用于结肠癌细胞侵袭性能相关的基础实验研究。

英文摘要：

Objective To screen an optimal medium for colon cancer cell invasion-related research. Methods Human colorectal cancer cell lines HCT-116, LOVO and CACO-2 were conventionally cultured in RPMI 1640, DMEM and DMEM-F12 mediums. The proliferations of HCT 116, LOVO and CACO-2 cells were measured by colony forming assay. The protein expressions of E-cadherin, vimentin, Nm23-H1 and p53 were determined by irnmunofluorescence, and the mRNA expressions of Nm23-H1, p53 and snail-1 were detected by RT-PCIL Results Compared with RPMI- 1640 and DMEM mediums, the rate of colony formation, protein expressions of vimentin and p53 and mRNA expressions of snail-1 and p53 were all obviously increased （P~0. 05）, while protein expressions of E-cadherin and Nm23-H1 and mRNA expression of Nm23-H1 were decreased in DMEM-F12 medium（P〈0. 05）. The rate of colony formation and expressions of cell invasion-related factors in RPMI1640 medium were not statistically different from those in DMEM medium （P〉0. 05）. Conclusion DMEM-F12 medium achieves a better result than DMEM and RPMI1640 mediums in the invasion-related research of colon cancer cells.