中文摘要：

高分子质量谷蛋白质亚基（HMW-GS）是决定小麦加工品质的重要因子,以25个小麦品种为材料,采用SDS-PAGE电泳方法研究亚基组成、各位点（Glu-A1、Glu-B1、Glu-D1）亚基积累量、谷蛋白大聚合体（GMP）含量及其与面筋含量的关系。研究结果表明：低蛋白小麦品种HMW-GS组成主要以＂N、7＋8/7＋9、2＋12＂亚基组合为主,高蛋白小麦品种HMW-GS组成则主要以＂1、7＋8/7＋9、5＋10＂亚基组合为主,中等蛋白小麦品种兼有以上两种类型亚基组合;亚基组成相同的小麦,籽粒GMP含量和蛋白质含量仍有较大差异。相关分析表明,HMW-GS亚基总积累量与籽粒蛋白质含量和GMP含量呈极显著正相关,与干、湿面筋含量成正相关;GMP含量与干、湿面筋含量、蛋白质含量均呈极显著正相关,说明小麦品质类型虽与HMW-GS组成有关,但亚基积累强度不同可能是导致籽粒品质类型差异的重要因素之一。

英文摘要：

Glutenin macropolyer（GMP） consists of high molecular and low molecular weight glutenin subunits（HMW-GS and LMW-GS）.HMW-GS plays an important role in exterminating the gluten quality in common wheat.The aim of this study was to analysis the main HMW-GS components,the accumulation of each subunit at different locus（Glu-A1,Glu-B1 and Glu-D1）,and GMP content for 25 wheat varieties by using SDS-PAGE,and to study their relationships with gluten content.Results： the components of HMW-GS in wheat grains with low-- protein content are usually N, 7 ＋8/7 ＋9, 2 ＋ 12, the frequency of subunit pairs N,7 ＋9, 2 ＋ 12 is mgn; while those with high protein content are usually 1,7 ＋ 8/7 ＋ 9, 5 ＋ 10, the frequency of subunit pairs 1,7 ＋ 8, 5 ＋ 10 is high; and those with medium protein content has the both types of subunit composition. Different wheat genotypes might have the same subunit components of HMW - GS, but their subunit accumulations are different. GMP and protein content in grains with the same HMW -GS subunits could still be significantly different. HMW -GS ac- cumulation amount is significantly and positively correlated with grain protein as well as GMP content, and positively correlated with gluten content. GMP content has a very significant positive correlation with gluten and protein contents. In conclusion, even though wheat grain quality for different end use is influenced by HMW - GS composition, HMW -GS accumulation amount might play a key role in affecting wheat grain quality.