Daidzein (7,4-dihydroxyisoflavone ) 是由修改 Atherton-Todd 反应的 phosphorylated。五个目标产品的结构,被 X 光检查,红外, NMR 和 ESIMS 决定。Electrospray 电离结果证明在煤气的阶段,所有 phosphorylated daidzein 衍生物能与蛋白质溶解酵素形成非共有原子价建筑群,当非共有原子价建筑群没与溶解酵素在 daidzein 的混合答案被检测时。每非共有原子价建筑群的相对亲密关系根据它的不同分解孔电压被获得。
Daidzein (7,4'-dihydroxyisoflavone) was phosphorylated by a modified Atherton-Todd reaction. The structures of the five target product, were determined by X-ray, IR, NMR and ESI-MS. Electrospray ionization results show that in the gas phase all the phosphorylated daidzein derivatives could form non-covalent complexes with the protein lysozyme, while non-covalent complexes were not detected in the mixed solution of daidzein with lysozyme. Relative affinity of every non-covalent complex was obtained according to its different decomposition orifice voltage.