中文摘要：

目的：探讨碘化N-正丁基氟哌啶醇（F2）对血管紧张素Ⅱ（AngⅡ）刺激血管平滑肌细胞（VsMCs）ERK1/2和cAMP反应元件结合（CREB）蛋白表达的影响。方法：原代培养大鼠胸主动脉VSMCs；用Western blot法检测ERKI12，p-ERKI／2，CREB和p-CREB蛋白表达。结果：AngⅡ可增加VSMCsp-ERK1／2和p-CREB蛋白表达，而不增加ERK1／2和CREB蛋白表达；F2（10^-8，10^-7和10^-6mol/L）剂量-依赖地降低AngⅡ刺激的p-ERKI／2和p-CREB蛋白表达。结论：F2抑制AngⅡ刺激VSMCs ERK1／2和CREB蛋白磷酸化，这可能是其抑制VSMCs增殖的机制。

英文摘要：

Objective： To investigate the inhibitory effects N-n-butyl halopeddol iodide（F2）on expression of ERK1/2 and cAMP response element binding （CREB）protein induced by angiotensin Ⅱ in rat vascular smooth muscle cells （VSMC3）. Methods： The primary rat thoracic aortic VSMCs were cultured in vitro. The levels of ERK1/2, p-ERK1/2, CREB and p-CREB were assessed by Western blot. Results： Ang II increased the expression of p-ERK1/2 and p-CREB but not ERK1/2 and CREB protein. F2 （10^-8, 10^-7 and 10^-6mol/L）concentration-dependenfly decreased the expression of p-ERK1/2 and p-CREB. Conclusion： F2 inhibits the phosphorylation of ERK1/2 and CREB protein, which may be the mechanisms of its inhibitory effects on the proliferation of VSMCs.