中文摘要：

对实验室保藏菌种进行筛选，得到一株葡萄汁酵母Saccharoinyces uvarum SW-58，能够催化还原4，4，4-三氟乙酰乙酸乙酯生成（R）-4，4，4-三氟-3-羟基丁酸乙酯。在优化条件下，摩尔转化率达到77．0％，产物的对映体纯度88．9％e．e．，产物浓度达到15．7g/L，产物浓度得到了显著的提高，达到优化前的3．3倍。

英文摘要：

Saccharoinyces uvarum SW - 58 is isolated from the microorganisms preserved in our laboratory. This strain catalyses enatioselective reduction of 4,4,4 - trifluoroacetoacetate. Under the optimum conditions the maximum concentration of （R） - ethyl 4,4,4 - trifluoro - 3 - hydroxybutanoate, the molar conversion and the optical purity reached 15.7 g · L^-1 , 77.0% and 88.9% e. e. , respectively, The product concentration is enhanced to 3. 3 times more than that under reduction condition before optimization.