中文摘要：

为探讨组蛋白去乙酰化酶抑制剂丙戊酸（valproicacid，VPA）对猪手工克隆（HMC）胚胎发育潜能的影响，本研究比较了不同浓度（0、25、50、75和100nmol／L）VPA处理猪HMC重构胚对后期胚胎发育率、内细胞团数和组蛋白乙酰化程度的影响。结果表明，50、75nmol／LVPA处理组HMC重构胚的卵裂率和囊胚发育率均显著高于0、25和100nmol／LVPA处理组（P〈0．05）；与0、25、75和100nmol／L相比，50nmol／LVPA处理组能显著提高囊胚内细胞团细胞数（P〈0．05），囊胚阶段VPA处理组的组蛋白H3K14乙酰化（AcH3K14）水平高于空白组和孤雌激活囊胚。因此，应用VPA处理可提高猪HMC重构胚胎分裂率、囊胚率及增加内细胞团细胞数，提高了猪HMC胚胎的体外发育潜能。

英文摘要：

To explore the effects of histone deaceylase inhibitors vaiproic acid （VPA） affecting the development potency of handmade cloned （HMC） embryos, porcine HMC reconstructed embryos were cultured for 24 h with different concentration （0,25,50,75 and 100 nmol/L） of VPA, and the blastocyst development rate, inner cell mass （ICM） cells number and histone acetylation level were compared. The results showed that reconstructed embryos were cultured in PZM-3 containing 50, 75 nmol/L VPA for 24 h, the frequency of the rate of cleavage and blastocyst formation were significantly higher in embryos trea- ted with VPA than 0, 25, 100 nmol/L VPA treatments （P〈0.05）. In addition, 50 nmol/L VPA treatment significantly in- creased the number of inner cell mass （ICM） cells compared with the other groups （P〈0.05）. The AcH3K14 level in embryos treated with VPA was higher than the control group and parthenogenetic activation group. In conclusion, epigenetic modifica tion VPA could enhances the rate of cleavage, blastocyst formation and the ICM ceils number of porcine HMC reconstructed embryos, which mean improve the developmental potency of HMC embryos.