中文摘要：

针对甲肝病毒的vp3/vp1壳蛋白区域设计引物,建立SYBR GreenⅠ实时荧光RT-PCR检测甲肝病毒的反应体系。此方法的病毒检测下限达到101TCID50,标准曲线为Ct=-3.052lg（TCID50）＋34.57,线形范围101～105TCID50,相关系数0.9961。该方法对甲肝病毒检测特异,与轮状病毒、腺病毒、诺如病毒、星状病毒无交叉反应。针对甲肝病毒标准品检测的批内试验变异系数（CV）为0.85%～1.71%（n=5）、批间试验CV为0.76%～1.98%（n=3）。运用此方法随机检测45份草莓样品,检测出3份阳性样品。该检测方法灵敏、特异、重复性好,可应用于水果和蔬菜中甲肝病毒的快速检测。

英文摘要：

A SYBR GreenⅠ real-time polymerase chain reaction （RT-PCR） method using the primers designed to encompassing the conservative region of vp3/vp1 in the HAV was developed for the detection of HAV in strawberry.The limit of detection of the method was 101 TCID50.The standard curve was Ct =-3.052 lg（TCID50 ）＋ 34.57,displaying good linearity over a linear range from 101 to 105 TCID50 with 0.9961 correlation coefficient.This method was specific to HAV without any cross-reaction with rotavirus,adenovirus,norovirus or astrovirus.The coefficients of variation （CV） for determining HAV standards with different titers by this method were between 0.85% and 1.71% （n = 5） in intra-assay and between 0.76% and 1.98% （n = 3） in inter-assay.Totally 45 strawberry samples were examined,and 3 of them were found positive.This method is sensitive,specific and reliable,and can be used for the rapid detection of HAV in fruits and vegetables.