中文摘要：

以1-乙基-3-（3-二甲基氨丙基）-碳化二亚胺和N-羟基琥珀酰亚胺为催化剂,将糖胺聚糖（肝素、硫酸乙酰肝素、硫酸软骨素A,B,C,D,E）中糖醛酸的羧基与氨基荧光素中氨基偶联,分别得到各荧光标记糖胺聚糖。将标记糖胺聚糖用点样仪点在硝酸纤维素包被玻璃芯片上,分别探讨其在硝酸纤维素薄膜上的保留率及其与硫酸软骨素抗体（克隆号CS-56）的相互作用。结果表明,各种糖胺聚糖在硝酸纤维素膜中保留率显著不同,硫酸软骨素E（51.7%）〉硫酸软骨素A（19.5%）〉硫酸乙酰肝素（16.4%）〉肝素（14.1%）〉硫酸软骨素B（12.3%）〉硫酸软骨素D（10.8%）〉硫酸软骨素C（10.7%）;定量计算得各种糖胺聚糖与CS-56结合能力：硫酸软骨素C〉硫酸软骨素D〉硫酸软骨素A〉硫酸软骨素E,硫酸软骨素B、肝素及硫酸乙酰肝素与CS-56无结合。

英文摘要：

The amino groups of aminofluorescein were covalently linked to the carboxyl groups of glycosaminoglycans（GAGs）（Heparin,Heparan sulfate,Chondroitin sulfate A,B,C,D and E） using 1-ethyl-3-（1,3-dimethylaminopropyl）-carbodiimide and N-hydroxysuccinimide as catalytic reagent,and the fluorescent labeled glycosaminoglycan was acquired,separately.The fluorescent labeled GAGs were printed on the nitrocellulose coated glass chips with a manual microarrayer.The retention rate of GAGs on nitrocellulose membrane was calculated after washing,and their interaction with monoclonal anti-chondroitin sulfate antibody（clone number CS-56） was further studied,separately.The experimental results showed that the retention rate was apparently different between GAGs,that is,chondroitin sulfate E（51.7%）chondroitin sulfate A（19.5%）heparan sulfate（16.4%）heparin（14.1%）chondroitin sulfate B（12.3%）chondroitin sulfate D（10.8%）chondroitin sulfate C（10.7%）.The interaction of GAGs with CS-56 was scientifically compared according to their retention rate on nitrocellulose glycoship,and after quantitatively calculation,the binding ability to CS-56 is： chondroitin sulfate CDAE,while chondroitin sulfate B,heparin and heparan sulfate did not show any interaction with CS-56.