中文摘要：

采用亲水作用色谱一高分辨电喷雾质谱联用技术（HILIC-HR-ESI／MS）结合麻痹性贝毒素（PSP）精确质量数数据库，建立快速筛查、定性识别赤潮藻中各种常见PSP的方法。产毒藻细胞破碎后经0．1mol／L乙酸溶液提取，通过HILIC-HR-ESI／MS正负离子模式全扫描分析，得到产毒藻粗提物中PSP化合物准分子离子及主要碎片离子精确质量数，结合PSP数据库筛查并辅以商品化软件数据分析，实现产毒藻所含PSP的快速定性识别。所建数据库包含25种常见PSP化合物精确分子质量及碎片离子信息，所发展的HILIC-HR-ESIfMS方法分离度高、灵敏度好，所考察的10种常见PSP化合物检测限在10-80nmol／L范围内，能满足产毒藻实际样品的分析要求。3种产毒藻实际样品筛查、分析结果良好，说明本方法是赤潮藻中麻痹性贝毒素快速筛选、定性识别的有效方法。

英文摘要：

A new method was developed for rapid screening and identification of paralytic shellfish poisoning （PSP） toxins in red tide algae by hydrophilic interaction chromatography-high resolution mass spectrometry （HILIC-HR-MS） combined with accurate mass database. Toxic algae sample was extracted with water contai- ning 0.1 mol/L acetic acid after cell-breaking, and then the crude extracts were analyzed by HILIC-HR-MS in positive and negative full scan modes. The accurate mass of quasi-molecular ion and main fragment ion peaks of PSP toxins in toxic algae were acquired and the PSP toxins in algae could be successfully qualitative identifi- cation by database screening and HR-MS data analysis using commercial software （ Mass Hunter）. The created database includes data not only the accurate relative molecular masses of 25 common PSP toxins but also the fragment ions. The results showed that the proposed HILIC-HR-ESI/MS method was sensitive and had the high separation efficiency. The limits of detection （LOD） of ten common PSP compounds were in the range of 10-80 nmol/L, and it could meet the requirements for the actual screening of toxic algae samples. The developed method was applied to screen the PSP toxins in three kinds of toxic algae which demonstrated that the method was a useful tool for the rapid screening and qualitative identification of common PSP toxins in harmful algae.