中文摘要：

目的探讨羰基-氰-对-三氟甲氧基本腙（FCCP）对脂肪酸结合蛋白3（FABP3）基因表达沉默的P19细胞线粒体功能的影响。方法构建FABP3表达沉默载体（A组）及相应的空载体（B组），包装病毒后感染P19细胞，建立稳定转染FABP3沉默的P19细胞株，采用实时定量PCR检测干扰效率。给予FCCP2．5〉mol／L（C组）、5〉mol／L（D组）干预FABP3沉默的P19细胞，采用荧光素酶化学发光法检测细胞ATP含量，流式细胞术观察线粒体膜电位的变化。结果B组FABP3基因表达量较A组下调（0．50±0．08vs．2．23±0．42）（P〈0．05）。与A、B组相比，C、D组细胞ATP生成减少（2．47±0．58、1．05±0．13vs．0．09±0．03、0．12±0．02）（P〈0．05），而细胞线粒体膜电位升高（358．36±12．23、389．66±12．13vs．437．35±9．27、473．21±19．65）（P〈0．05）。结论FABP3基因在维持P19细胞线粒体功能中发挥重要作用。

英文摘要：

Objective To investigate the effect of cyanide p-trifluoromethoxyphenyl-hydrazone （FCCP） on mitochondrial function of FABP3-silenced P19 cells. Methods The silencing vector of FABP3 in group A and the corresponding empty vector in group B were constructed and packaged with lentivirus and then transfected into P19 cells. After FABP3-silenced P19 cells were established, the efficiency to knockdown was detected by RQ-PCR. Beside.s, FABP3-silenced P19 cells were treated with FCCP 2.5 μmol/L in group C and 5μmol/L in group D, respectively. ATP production in P19 cells was determined by luciferase-based luminescence assay and mitochondrial membrane potential was measured by flow cytometry. Results The mRNA expression of FABP3 in group B was lower than that in group A（0.50±0. 08 vs. 2. 23±0. 42）（P〈0. 05）. Compared with groups of A and B, the total ATP production in P19 cells was decreased（2.47±0. 58 and 1.05±0. 13 vs. 0. 09±0. 03 and 0. 12± 0. 02）（P〈0. 05）, while mitochondrial membrane potential was increased（358. 36± 12.23 and 389. 66 ±12. 13 vs. 437. 35 ± 9. 27 and 473. 21 ± 19. 65） in groups of C and D（P〈0. 05）. Conclusion FABP3 may play an important role in the maintenance of mitochondrial function in P19 cells.