中文摘要：

目的建立气相色谱-质谱联用快速检测粪便中短链脂肪酸的方法,并探讨将其应用于肠道疾病辅助诊断的可能性。方法分别取健康C57BL/6小鼠、SD大鼠和成年人志愿者粪便,以及用硫酸葡聚糖溶液诱导的肠炎模型C57BL/6小鼠的粪便;用气相色谱-质谱联用法检测上述标本中短链脂肪酸的种类和相对丰度。结果所有标本均检测出多种短链脂肪酸,其种类主要有乙酸、丙酸、丁酸和戊酸。在生理条件下,人与鼠短链脂肪酸的种类和相对丰度比都十分相似。肠炎可使粪便中除乙酸外的其他短链脂肪酸的相对丰度下降,其中,尤以丁酸下降最为明显。结论气相色谱-质谱联用法可快速检测粪便中的短链脂肪酸,并有可能应用于肠炎等肠道疾病的辅助诊断。

英文摘要：

Objective To establish a method to detect short-chain fatty acids （SCFAs） in feces rapidly by gas chromatography-mass spectrometry （ GC-MS）, and evaluate its potential roles in the auxiliary diagnosis of intestinal diseases. Methods Feces samples from healthy volunteers, SD rats and C57BL/6 mice with or without dextran sulfate induced colitis were collected, and the types and relative abundance of SCFAs in these samples were analyzed by GC-MS. Results All feces samples could be detected several kinds of SC- FAs, such as acetic acid, propionic acid, butyric acids and pentanoic acid. Under the physiological condition, there were similar types and relative abundance of SCFAs in feces between human and mice or rat. However, colitis might decrease the relative abundance of SCFAs especially butyric acid, but except acetic acid. Conclusion The SCFAs in feces can be detected rapidly by using GC-MS, which may be helpful for the diagnosis of intestinal diseases.