中文摘要：

目的评价骨髓间充质干细胞（BMSCs）和纤维蛋白胶（FG）作为种子细胞和支架材料修复大鼠牙槽骨缺损的效果。方法 30只SD大鼠随机分成A、B组,建立SD大鼠上颌牙槽骨缺损模型,联合植入BMSCs和FG复合物,A组对照侧单纯植入FG,B组对照侧为空白对照。利用上颌骨实验区切片苏木精-伊红染色及Micro-CT扫描观察成骨效应。结果苏木精-伊红染色切片显示：术后3周可见实验侧骨小梁形成,对照侧无明显骨修复;术后6周可见实验侧新骨形成增加,对照侧只有纤维组织修复。Micro-CT扫描表明实验侧成骨修复明显优于对照侧。结论应用BMSCs联合FG可较好地修复大鼠牙槽骨缺损,是修复小范围骨缺损理想的种子细胞与支架材料。

英文摘要：

Objective To explore the effects and feasibility of the repairing alveolar bone by combination of bone marrow stromal cells（BMSCs） and fibrin glue（FG）.Methods BMSCs were obtained from the sucking mouse and then separated,cultured to the second generation to get ready for use.The available cells were combined with FG and then immediately implanted into the defect that had been established in one lateral maxilla of 30 SD rats.At the same time pure FG without BMSCs was implanted into the other side.The rats were killed at the 3rd and 6th week after operation.The hematoxylin-eosin staining and Micro-CT were used to observe and evaluate the osteogenesis.Results New bone trabecula formed at experiment side of the rats at 3rd week.The repairing seemed better after 6th week of operation than the control side.There were only fibrous tissues in the control side or none.Conclusion This experiment showed a feasible approach of repairing the defects in alveolar bone of the rats.