黑花生花色苷具有很好的应用前景,为建立一个快速检测花生花色苷的方法,以黑花生和普通花生的种皮为材料,利用高效液相色谱测定了黑花生和普通花生种皮中花色苷的含量和主要成分。研究表明,黑花生种皮中花色苷的总含量为1.16mg/g鲜重。在高效液相测定过程中,其主要成分在保留时间10.283min和11.217min处有2个高吸收峰,它们的含量占总花色苷含量的98.3%。根据已有的研究结果,推断二者分别为矢车菊素-3-槐二糖、矢车菊素-3-接骨木二糖,其含量分别比普通花生高810倍和580倍。
In order to establish a simple method to determine anthocyanin content of black peanut,which has great commercial potentials,we measured the content and major compositions of anthocyanin in both ordinary and black peanut testae by HPLC in this paper.Our results showed that there was 1.16mg/g total anthocyaninin fresh back peanut testae.Two major components,the content of which was 98.3%of total anthocyanin inback peanut testa,were detected as two peaks at 10.283 min and 11.217 min respectively during HPLC.They were 810 times and 580 times higher than ordinary peanuts’respectively,which were deduced as cyanidin-3-sophoroside and cyanidin-3-sambubioside according to previous study.