中文摘要：

采自湖南地区的辣椒轻斑驳病毒Pepper mild mottle virus（PMMoV）样品经单斑分离后,根据已经报道的PMMoV序列基因保守区设计6对简并引物,采用片段重叠法和RACE方法扩增、克隆获得一个全长为6 356bp的湖南分离物（PMMoV-HN1,登录号：KP345899）全基因组序列,编码4个蛋白,分别为126kD蛋白（70~3 423nt）、183kD蛋白（70~4 908nt）、28kD蛋白（4 909~5 682nt）和17.5kD蛋白（5 685~6 158nt）,5′-非编码区（5′-UTR）和3′-非编码区（3′-UTR）分别含有69和198个碱基,其中5′-UTR存在一个序列为m7G5′pppG的甲基化核苷酸帽子结构。一致性分析发现PMMoV-HN1与PMMoV其他分离物的核酸一致性为94%~99%,编码的氨基酸一致性为94%~99%。全基因组序列系统进化分析表明PMMoV-HN1分离物与中国首次报道的PMMoV-CN分离物亲缘关系最近。本研究是国内报道的第二例PMMoV全基因组序列。

英文摘要：

Pepper samples were collected from Hunan,and identified as Pepper mild mottle virus（PMMoV）.According to the previously reported gene conserved regions of PMMoV,six pairs of degenerate primers were designed.The 6 356 bp whole-genome sequence（PMMoV-HN1,accession no.KP345899）was obtained.There were four open reading frames,encoding four proteins,including 126 kD protein（70-3 423 nt）,183 kD protein（70-4 908 nt）,28 kD protein（4 909-5 682 nt）and 17.5 kD protein（5 685-6 158 nt）.The 5′-non-coding region（5′-UTR）and the 3′-non-coding region（3′-UTR）contained 69 and 198 nucleotides,respectively,in which5′-UTR had a sequence of m7G5′pppG methylation in the nucleotide cap structure.Identity analysis found that the nucleotide identity of PMMoV-HN1 and other PMMoV isolates was 94%-99%,and the identity of the encoded amino acids was 94%-99%.Phylogenetic analysis showed the closest phylogenetic relationships between PMMoV-HN1 and PMMoV-CN.