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条斑紫菜壳孢子的抗生素敏感性研究
  • ISSN号:1674-5566
  • 期刊名称:上海海洋大学学报
  • 时间:2012.11.11
  • 页码:982-988
  • 分类:S948[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]上海海洋大学水产与生命学院,上海201306
  • 相关基金:国家高技术研究发展计划(2012AA10A411);国家自然科学基金(31072208);农业部公益性专项(200903030);国家农业科技成果转化资金项目(2011GB2C000005);上海市科学技术委员会重点科技攻关项目(10391901100);国家海洋局公益专项(201105008,201105023);上海市教育委员会海洋生物学重点学科建设项目(J50701)
  • 相关项目:野生坛紫菜的性别与性别决定机理研究
中文摘要:

以往研究紫菜细胞对抗生素的敏感性时,常以叶状体的酶解单离体细胞为材料,但由于来自同一个叶状体的体细胞处于不同的分化阶段,离体培养时,体细胞的成活、再生和发育途径均不相同,从而无法准确地判断抗生素对紫菜离体细胞的生长和发育影响。以成活、萌发和发育途径等几乎一致的条斑紫菜壳孢子为研究材料,较系统地观察了3种抗生素对壳孢子的成活、生长和发育的影响。结果表明:卡那霉素能促进条斑紫菜壳孢子的萌发、生长和发育。加卡那霉素培养7d,26%的壳孢子萌发体,其细胞数多于20个,比对照组多2倍,培养11d和18d,萌发体的平均长度分别为对照组的1.6倍和2.1倍。氨苄青霉素能显著地抑制条斑紫菜的壳孢子萌发、生长和发育,造成细胞的分裂速度减缓、叶状体发育异常。加氨苄青霉素培养7d,80%的壳孢子萌发体的细胞数少于15个,培养11d和18d,各试验组的壳孢子萌发体的平均长度均显著低于对照组,并且随着氨苄青霉素浓度的提高,萌发体的平均长度下降。氯霉素对条斑紫菜的壳孢子具有强烈的致死作用。加氯霉素培养7d,浓度大于100μg/mL的各试验组的壳孢子萌发体全部死亡;培养11d,低浓度组(50μg/mL)的萌发体也全部死亡,90%以上的萌发体的细胞数小于5个。由此得出,氯霉素是开展条斑紫菜基因工程的理想抗性选择压力。

英文摘要:

In the previous studies on the antibiotic sensitivity of Porphyra,the somatic cells isolated enzymatically from Porphyra gametophytic blades were often used.However,the accurate analysis of antibiotics effects on the survival,growth and development of the somatic cells was difficult,because the somatic cells isolated even from a Porphyra blade had different regeneration capacity,cell division and developmental pathways when they were cultured in vitro.In this paper,the antibiotic sensitivities of Porphyra yezoensis conchospores which are almost the same in viability,germinability and developmental pathways,were studied by treatment with kanamycin,ampicillin and chloramphenicol.The obtained results demonstrated that kanamycin could promote the growth and development of conchospore germlings.After 7 days of culture,the cell number of 26% of the conchospore germlings was over 20 cells,which was twice as many as that of control group.After 11 and 18 days of culture,the mean length of conchospore germlings was 1.6 times and 2.1 times longer than that of control group,respectively.The treatment with ampicillin could inhibit the growth and development of conchospore germlings,showing decrease of cell division rates and inhibitation of germling development.After 7 days of culture,the cell number was less than 15 in 80% of conchospore germlings.After 11 and 18 days of culture,the mean length of conchospore germlings was shorter than that of control group,as ampicillin concentrations increased.The chloramphenicol had strong lethal effect on conchospore germlings.When chloramphenicol concentration was higher than 100 μg/mL,the conchospore germlings died out after 7 days of culture.The conchospore germlings treated with 50 μg/mL of chloramphenicol also died out after 11 days of culture.The cell number was less than 5 cells in 90% of the dead conchospore germlings.Therefore,it was concluded that the chloramphenicol is an effective selective pressure in genetic engineering of P.yezoensis.

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期刊信息
  • 《上海海洋大学学报》
  • 北大核心期刊(2011版)
  • 主管单位:上海市教委
  • 主办单位:上海海洋大学
  • 主编:陈裕东
  • 地址:上海市临港新城沪城环路999号
  • 邮编:201306
  • 邮箱:xuebao@shfu.edu.cn
  • 电话:021-61900229
  • 国际标准刊号:ISSN:1674-5566
  • 国内统一刊号:ISSN:31-2024/S
  • 邮发代号:4-604
  • 获奖情况:
  • 曾获《CAJ-CD规范》执行优秀奖,水产类期刊二等奖,全国优秀农业期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:3383