中文摘要：

目的：比较罗库溴铵或顺式阿曲库铵24 h持续输注对机械通气SD大鼠膈肌功能的影响。方法：雄性SD大鼠随机分为四组：一组作为对照组,另外三组用来进行24 h的机械通气。其中一组为罗库溴铵组持续输注罗库溴铵,一组顺式阿曲库铵和一组生理盐水。结果：24 h机械通气后,与生理盐水组相比,罗库溴铵组的膈肌最大强直张力下降29%,膈肌MuRF-1的mRNA水平增加30%,calpain活性增加约57%,而等效剂量的顺式阿曲库铵组则没有明显改变。结论：与顺式阿曲库铵相比,罗库溴铵可引起Calpain活性的增强和泛素-蛋白酶体系统的激活,对膈肌功能的损害和蛋白水解活性的增强作用更明显。

英文摘要：

Objective：The aim of our study was to compare the effects of 24 h infusion of rocuronium or cisatracurium on the diaphragm in mechanically ventilated SD rats.Methods：Male SD rats were randomly divided into four groups：a control group（CON） and three groups submitted to controlled mechanical ventilation for 24h.Among them,MVR group received a continuous infusion of rocuronium,while MVC with cisatracurium and MVS with 0.9% NaCl.Results：After 24h mechanical ventilation,in vitro diaphragm tetanic force was decreased after rocuronium（-29 % vs.saline）,while the force was more preserved after equivalent dose infusion of cisatracurium.Diaphragmatic MuRF-1 mRNA was increased after rocuronium（＋30 %vs.saline）,while unchanged in cisatracurium group.Calpain activity was increased after rocuronium（＋57 % vs.saline） and unchanged in the cisatracurium groups.Conclusion：Cisatracurium infusion during controlled mechanical ventilation exerted less detrimental effects on diaphragm function and proteolytic activity than infusion of rocuronium.These data suggest that increased calpain activity and increased activation of the ubiquitin proteasome system play a role in the different effects of these agents.