中文摘要：

为揭示菊黄东方鲀幼鱼转食过程中营养特点,提高转食期成活率,采用生化分析手段对驯化转食前、中、后的菊黄东方鲀幼鱼生长和全鱼脂肪酸组成及含量进行了检测和分析。结果显示,菊黄东方鲀幼鱼在驯化完全转食配合饲料后,呈现出体质量的生长补偿现象。驯化前幼鱼的肥满度最高（10.02）,在驯化中最低（5.43）,驯化后肥满度有显著恢复（6.00）。幼鱼摄食配合饲料后,全鱼的干物质含量明显升高。在菊黄东方鲀驯化转食前、中、后阶段的干样中检出8种饱和脂肪酸（SFA）、7种单不饱和脂肪酸（MUFA）和12种多不饱和脂肪酸（PUFA）。从百分含量上看,幼鱼驯化转食过程中,C16：0含量最高且稳定（21.26%-23.81%）,C18：0含量显著下降,C16：1和C18：1n9c含量显著上升;C22：6n3（DHA）含量较丰富（11.95%-13.05%）,且呈现略微降低;C20：5n3（EPA）在驯化中略微下降,驯化后又略有上升;C22：5n3（DPA）呈现显著降低。SFA的比例相对稳定（38.13%-42.52%）,MUFA的比例呈现显著上升趋势,但PUFA的比例（特别是∑n3PUFA）呈现显著下降趋势。值得注意的是,配合饲料的C18：2n6c含量特别高（21.29%）,但摄食配合饲料的幼鱼体内的C18：2n6c含量非常低（2.47%）;相反,配合饲料的C16：1含量特别低（3.87%）,但摄食配合饲料的幼鱼体内的C16：1含量较高（9.25%）。研究表明菊黄东方鲀幼鱼后期对n3PUFA（特别是DHA和EPA）有较高的需求量,但不需要过多的C18：2n6c,部分的C16：1需要从其他营养物质转化过来。为提高菊黄东方鲀幼鱼驯化转食配合饲料过程中成活率,建议：（1）幼鱼驯化转食前,加强投喂活体饵料,特别是富含n3PUFA的海水桡足类;（2）幼鱼驯化转食的配合饲料中加大DHA和EPA的添加量,同时减少C18：2n6c比例、增加C16：1的比例。

英文摘要：

In order to understand the the nutritional requirement of juvenile tawny puffer （Takifugu flavidus） during the bait conversion period, the growth, body lipid content and its fatty acid composition of tawny puffer juvenile before, during, and after the domestication were detected and analyzed by biochemical analysis methods. The results show that the phenomenon of body weight compensatory growth of tawny puffer juvenile has been found after the domestication; the highest condition factor of juvenile was found before the domestication （10.02）, the lowest condition factor was found during the domestication （5.43）, and it increased significantly after the domestication （6. 00 ）. The dry matter contents of tawny puffer juvenile increased significantly after the domestication. 8 saturated fatty acids （SFA）, 7 mono-unsaturated fatty acids （MUFA）, and 12 poly-unsaturated fatty acids （PUFA） were found in the dry sample before, during, and after the domestication. From the percent content, during the bait conversion period, the C16：0 content of tawny puffer juvenile was the highest and stable （21. 26% -23. 81% ）, the C18：0 content decreased significantly, and the C16：1 and C18：1n9c contents increased significantly; the C22：6n3 （DHA） content was rich （11.95% -13.05% ）, it decreased faintly during the bait conversion period; the C20：5n3 （EPA） percent content of juvenile decreased faintly during the domestication, and increased faintly after the domestication; the C22：5n3 （DPA） percent content decreased significantly during the bait conversion period. During the bait conversion period of tawny puffer juvenile, the SFA was relatively stable （38. 13% - 42.52% ）, the MUFA increased significantly, however, PUFA decreased significantly, especially ∑n3PUFA. And more notably, the C18：2n6c percent content of the compound feed was particularly high （21.29%）, however, the C18：2n6c content of juvenile fed with compound feed was very low （2.47%） ;