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中文摘要：
在碱性介质中,四环素能将紫红色的高锰酸钾还原成绿色的锰酸钾。基于这一反应,提出了测定四环素的新方法。实验发现该体系在526 nm和608 nm分别有吸收谷和吸收峰,本实验以这两个波长为测量波长,以360 s为反应时间,对实验条件进行了考察。在最优实验条件下,测得四环素的线性范围为0.5-7.5 mg.L-1,检测限为0.23 mg.L-1。将该方法应用于药物中四环素的测定,结果表明与已有的测定方法（高效液相色谱法、直接电位法、荧光光度法等）相比具有相当的检测限和灵敏度。
英文摘要：
A simple and sensitive kinetic spectrophotometric method was proposed for the determination of tetracycline in pharmaceutical preparation samples.The method was based on the reduction of violet potassium permanganate by tetracycline in the alkaline medium at 45 ℃ to form green potassium manganate.It was found that,in this kinetic reaction,the absorbance at both 608 nm and 526 nm linearly changed in the range of 0.5 to 7.5 mg·L-1,with the limit of detection of 0.23 mg·L-1 and 0.27 mg·L-1 respectively.The proposed method was applied to determination of tetracycline in pharmaceutical preparation samples with satisfactory results,which compared well with those obtained by HPLC.
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