采用便携式拉曼光谱仪对新鲜宫颈癌组织、宫颈上皮内瘤变Ⅲ级( CIN Ⅲ)组织及正常宫颈组织进行检测,通过光谱特征峰分析比较了各组织中化学成分的差异,归纳了3类组织的拉曼光谱特征及区别。正常组织以脂类特征峰(817,1127,1176,1450,1769 cm-1)为主,而宫颈癌和宫颈上皮内瘤变Ⅲ级组织则以蛋白特征峰(755,1003,1372,1542,1577 cm-1)为主。病变组织的主要区别在于宫颈上皮内瘤变Ⅲ级组织在853和1542 cm-1处出现了较明显的蛋白特征峰;宫颈癌组织则含有明显的核酸特征峰(784,1094,1345 cm-1)。通过特征峰归属及分析发现,3类组织在磷酸二酯基团形成氢键的能力、 DNA的相对含量、亚甲基的无序性、酰胺Ⅰ带的C O变形振动及类胡萝卜素的有无等方面存在显著差异。表明拉曼光谱可检测宫颈癌组织,探索宫颈癌与宫颈上皮内瘤变Ⅲ级的联系与区别,以早期诊断宫颈癌,具有良好的临床应用潜力。
A portable Raman spectrometer was used for distinguishing the characteristics of cervical cancer, cervical intraepithelial neoplasias Ⅲ(CIN Ⅲ)and normal fresh cervical tissues. Based on spectral profiles, the presence of lipids(817, 1127, 1176, 1450, 1769 cm-1)is indicated in normal tissue, and proteins(755, 1003, 1372, 1542, 1577 cm-1)are found in diseased tissues. Between CIN Ⅲ and cervical cancer tissues, proteins(853 and 1542 cm-1)and nucleic acids(1340 cm-1)are found to be good discrimination parameters. These three kinds of tissues have significant differences on the ability of forming hydrogen bond between di-hydrogen phosphate ester groups, the relative content of DNA, the disorder of methylene, the deformation of amide I band CO and the formation of carotenoids. These features show that Raman spectroscopy has good clinical application potential which can be used to detect cervical cancer tissues, explore the relationships and differences between cervical cancer and CIN Ⅲ and diagnose cervical cancer early.