中文摘要：

为了解蜱吸血和病原传播的分子机制，本研究从亚洲璃眼蜱唾液腺中克隆获得一个编码免疫抑制蛋白P36的同源性基因，该基因全长924bp，编码区为708bp，预测的编码蛋白包含235个氨基酸，分子量为27kuBLAST分析表明，该基因预测的氨基酸与美洲花蜱和安氏革蜱唾液腺中的免疫抑制蛋白P36高度同源RT—PCR分析表明，该基因在未吸血成蜱中没有表达，但吸血后表达、将目的基因与载体pGEX-4T-1连接后转化BL21表达菌，经诱导后得到GST融合重组蛋白：该实验结果为进一步研究P36奠定了基础。

英文摘要：

To understand the molecular mechanism of tick blood feeding and pathogen transmission, we cloned a full-length sequence of the immunosuppresant protein P36 gene from Hyalomma asiaticum tick. The cloned gene was 924 bp in length containing an ORF of 708 bp which encodes 235 amino acids. Blast analysis showed the gene shared significant homology with the immunosuppresant protein P36 of Dermacentor andersoni and Amblyomma americanum. The mRNA transcripts of P36 could be detected in the fed tick, but not in unfed tick. The gene was subcloned into expressing vector pGEX-4T-lafter removing the signal peptide and expressed successfully as a GST fusion protein.