中文摘要：

目的 观察三七注射液对阿霉素肾纤维化大鼠肾组织转化生长因子-β（1TGF-β1）和P38 MAPK的影响。方法 120只Sprasue-Dawley大鼠随机分为5组：正常组（=24）、假手术组（=24）、模型组（=24）、三七注射液组（=24）和三七总皂苷组（=24）,12周后处死大鼠。免疫组化和Western blot观察大鼠肾组织TGF-β1和P38 MAPK蛋白的表达。结果 正常组和假手术组大鼠肾小管可见极少量的TGF-β1和P38 MAPK阳性蛋白表达;而模型组大鼠肾组织TGF-β1和P38 MAPK阳性蛋白表达上调,与假手术组相比较,差异均有统计学意义（P〈0.01）;与模型组相比较,三七注射液组和三七总皂苷组大鼠肾组织,TGF-β1和P38 MAPK蛋白表达明显下调,差异均有统计学意义（P〈0.01）。结论 三七注射液的肾保护作用可能与下调TGF-β1和P38 MAPK水平,抑制TGF-β1/P38 MAPK信号通路活化有关。

英文摘要：

[ Objective ] To observe the effect of SanQi injection on the protein expression of Transforming Growth Factor-β1 （TGF-β1）, P38 MAPK in the renal tissue of rat model with Adriamycin-induced kidney fibrosis. [Methods] A total of 120 Sprasue-Dawley rats were randomly divided into five groups： normal group （n = 24）, sham group （n = 24）, model group （n = 24）, SanQi injection group （n = 24） and panax notoginseng saponins （PNS） group （n = 24）. All the rats were sacrificed after 12 weeks, and the protein expressions of TGF-β1 and P38 MAPK in the renal tissue were detected by immunohistochemistry and Western blot. [Results] The TGF-β1 and P38 MAPK were slightly expressed in the normal group and sham group. However, the protein expressions of TGF-β1 and P38 MAPK in the model group were significantly higher than those in the sham group （P 〈 0.01）. The protein expressions of TGF-β1 and P38 MAPK in both SanQi injection group and PNS group were significantly lower than those in the model group（P 〈 0.01）. [Conclusions] SanQi injection could provide a nephroprotective role by down-regulating the protein expressions of TGF-β1 and P38 MAPK, and blocking the activation of TGF-β1/P38 MAPK signaling pathway.