中文摘要：

用光刻法在普通定量滤纸上制作出了疏水图案，得到了滤纸微孔板。将表面修饰后的SiO，微球负载在滤纸表面，以提高滤纸纤维吸附蛋白质的能力，基于此建立了以间接酶联免疫吸附法测定羊抗兔免疫球蛋白的方法。以酶标兔抗羊IgG为例，负载SiO，微球后，滤纸微孔板吸附该蛋白质的量提高了20％～700％；将SiO2负载法应用于羊抗兔lgG的检测时，可使信号增强20％～150％。在本实验条件下，测定羊抗兔IgG的线性范围为3×10～3×10-8mmol孔。本方法使生物免疫试剂消耗量减少至3～5μL／孔、检测时间降至20min，板、滤纸微孑L板制作成本可低至约每板1．1美元，为研制准确、快速和低成本的疾病检测设备提供了新的思路和可能性。

英文摘要：

Photolithography was used to pattern hydrophobic design on common quantitative filter paper, based on which we successfully fabricated the paper-based micro-zone plates. In order to improve its protein- absorbing capability for further signal magnification, modified silicon dioxide （SiO2 ） micro-beads were deposited onto the surface of paper. Then indirect-ELISA for goat anti-rabbit IgG was applied on the 36-well plates. Taking enzyme linked goat anti-rabbit IgG as an example, loading SiO2 beads could remarkably increase the quantity of the absorbed protein by 20% -700%. When this method was used for goat anti-rabbit IgG detection, signals were magnified by 20% -150% , and the linear range of detection was from 3 x 10-~2 mmol/zone to 3 x 104 mmol/zone under given conditions. In this manner, bio-reagent consumption was reduced to 3-5 pL per zone, test time dropped to 25min per plate and the cost of fabrication reduced to S1.1 per plate, which indicated a promising application foreground for new disease diagnosis devices.