中文摘要：

目的探讨有机溶剂三氯乙烯（trichloroethylene，TCE）、四氯乙烯（perchloroethylene，PCE）对体外培养的皮肤角质形成细胞（keratinocyte，KC）的脂质过氧化的作用。方法利用中性红吸附（neutral red uptake，NRU）实验确定TCE和PCE对人KC的中性红半数吸收浓度（50％ neutral red uptake，NR50），并根据此确定染毒浓度。分别使用2．0、1．0、0．5、0、25、0．125mmol／L的TCE或0．8、0．4、0．2、0．1、0．05mmol／L的PCE对体外培养的人KC染毒4h，利用MDA、SOD和ROS试剂盒测定细胞中MDA、SOD和ROS水平。结果TCE、PCE对人KC的NR劬分别为4.53mmol／L和2.16mmol／L。经不同浓度TCE、PCE作用4h后，细胞内MDA水平和ROS水平随TCE或PCE浓度增加呈上升趋势，而SOD活性呈下降趋势，与溶剂对照组相比较差异有显著性，且存在浓度．反应关系。结论TCE、PCE对人KC有氧化损伤作用。

英文摘要：

Objective To explore the effects of different concentrations of trichloroethylene （TCE） and perchloroethylene （PCE） on lipid peroxidation in cultured human keratinocyte （KC）. Method Neutral red uptake assay was used to determine the NR50 of TCE and PCE, thereby the exposure concentrations of TCE were ascertained as 2.0, 1.0, 0.5, 0.25 and 0.125 mmol/L, and the concentrations of PCE were ascertained as 0.8, 0.4, 0.2, 0.1 and 0.05 mmol/L, which were respectively cultured with KC for 4h; then the levels of MDA, SOD and ROS in cultured cells were determined with special kits. Result It was shown that the NR50 of KC to TCE and PCE were 4.53 mmol/L and 2.16 mmol/L respectively, and the levels of MDA and ROS in cultured cells were gradually risen with the concentration of ＂ICE or PCE used four hours after exposure to TCE or PCE. Conclusion TCE or PCE could induce oxidative damage to cultured KC.