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CD8^+调节性T细胞在ITP发病中的意义及大剂量地塞米松的影响
  • ISSN号:1004-2806
  • 期刊名称:《临床血液学杂志》
  • 分类:R558.2[医药卫生—血液循环系统疾病;医药卫生—临床医学;医药卫生—内科学]
  • 作者机构:山东大学齐鲁医院血液内科,济南250012
  • 相关基金:国家自然科学基金(No:81170475,81470285)
中文摘要:

目的:研究大剂量地塞米松治疗前后免疫性血小板减少症(ITP)患者体内CD8^+ 调节性T细胞(Treg)及相关细胞因子的变化,了解CD8^+ Treg在ITP发病中的意义及大剂量地塞米松的影响。方法:流式细胞仪分析初诊ITP患者大剂量地塞米松治疗前后CD8^+ Treg的变化,ELISA法检测患者治疗前后血浆转化生长因子(TGF)-β1和白细胞介素(IL)-10的水平。结果:治疗前ITP患者CD8^+ T细胞亚群中的CD8^+ CD28^-Treg,尤其是CD8^+ CD25^+ Foxp3^+ Treg细胞比例较正常对照明显下降,血浆TGF-β1和IL-10的水平明显降低;大剂量地塞米松治疗后,获得缓解的ITP患者的CD8^+ CD28^- Treg及CD8^+ CD25^+ Foxp3^+ Treg细胞比例较治疗前明显升高,而与正常对照比较差异无统计学意义;血浆TGF-β1和IL-10的水平亦明显升高。结论:在初诊ITP患者CD8^+ T细胞亚群中,CD8^+ Treg细胞比例减少,获得缓解后CD8^+ Treg细胞比例增加,说明CD8^+ Treg参与了ITP的发病过程,增加CD8^+ Treg细胞比例和上调血浆TGF-β1和IL-10的水平可能是大剂量地塞米松发挥作用的机制之一。

英文摘要:

Objective:To investigate the change of CD8^+ regulatory T cell (Treg) and cytokines before and after treatment with high-dose dexamethasone,and assess the role of CD8^+ Treg and the effect of high-dose dexamethasone in patients with immune thrombocytopenia (ITP). Method: The cell numbers of CD8^+ Treg were analyzed by flow cytometry. The plasma levels of TGF-β1 and IL-10 were measured by ELISA. Result: The percentage of CD8^+CD28^- Treg, especially CD8^+ CD25^+ Foxp3^+ Treg was significantly decreased in ITP patients when compared with controls,and the plasma levels of TGF-β1 and IL-10 were significantly lower compared with controls. After high-dose dexamethasone treatment, the percentages of CD8^+ CD28^- Treg and CD8^+ CD25^+ Foxp3^+ Treg were significantly higher than that in ITP patients with remission. The plasma levels of TGF-β1 and IL-10 in patients with remission were also increased after treatment. Conclusion: These findings suggest that reduced CD8^+ Treg might contribute to the pathogenesis of ITP, and that elevated level of TGF-β1 and IL-10 and up-regulated CD8^+ Treg might contribute to the therapeutic effect of high-dose dexamethasone in the treatment of ITP.

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期刊信息
  • 《临床血液学杂志》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国教育部
  • 主办单位:华中科技大学同济医学院附属协和医院 北京大学医学院血研所
  • 主编:宋善俊 陆道培 胡丽华
  • 地址:武汉市解放大道1277号
  • 邮编:430022
  • 邮箱:lcxyxzz@qq.com
  • 电话:027-85726342-8806
  • 国际标准刊号:ISSN:1004-2806
  • 国内统一刊号:ISSN:42-1284/R
  • 邮发代号:38-169
  • 获奖情况:
  • 中国科技论文统计源期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊
  • 被引量:7788