研究了青藏高原东缘3种灌木种子(山梅花Philadelphus incanus、高山绣线菊Spiraea alpina和南川绣线菊S.rosthornii)经过0、40、80和160 d 5℃冷藏后,在5℃/25℃、12h/12 h光/暗培养下的萌发特性以及室温干储120 d后在白光、黑暗、红光、蓝光和12 h/12 h白光/黑暗(变光)5个水平,10℃/25℃变温培养下的萌发特性。结果表明:冷藏时间对3种灌木种子的萌发率和萌发速率均有极显著的影响,冷藏能提高山梅花和南川绣线菊种子的萌发能力,而不利于高山绣线菊种子的萌发;光照对山梅花种子萌发率有极显著的影响,红光和白光/黑暗培养下的萌发率最高,萌发速率最快,黑暗和蓝光培养条件下的种子萌发率最低,萌发速率最慢;光照对南川绣线菊种子萌发率和萌发速率有显著影响,白光、红光、蓝光、白光/黑暗培养下种子的萌发率均在80%以上,明显高于黑暗条件下,但红光下种子的萌发速率最快,蓝光下的萌发速率最慢;不同光照下高山绣线菊种子萌发率没有差异,均在93%以上,但红光和黑暗条件下种子的萌发速率最快,蓝光下的萌发速率最慢。本实验结果表明,冷藏作为一种打破种子休眠的常用手段,其作用因种而异;红光作为萌发的促动因子,其对各物种作用较为一致。
The fresh mature seeds of three shrubs (Philadelphus incanus, Spiraea alpine and S. rosthornii) were collected from the eastern Qinghai-Tibet Plateau. After the seeds were stored at 5 ℃ for different time (0, 40, 80 and 160 d), they were used for germination under 5℃/25 and 12 h/12 h photoperiod in a growth incubator. In addition, the seeds naturally dehydrated at room temperature for 120 d were used for germination at 10 ℃/25℃ under five light conditions (white light, dark, red light, blue light, 12 h/12 h light/dark). Cold storage time had signifi- cant effects on the germination of the seeds of the three shrub species. Cold storage improved the germination ability of P. incanus and S. rosthornii, but reduced the germination ability of S. alpine. There were very significant differences in germination percentage among different light conditions for P. incanus seeds. The highest germination percentage and fastest germination rate were recorded under red light and 12 h/12 h light/dark, and the lowest germination percentage and slowest germination rate were found under dark and blue light. Seeds of S. rosthornii exhibited significant differences in germination percentage and germination rate among the different light conditions. The germination percentages under white light, red light, blue light, and 12 h/12 h light/dark were all more than 80%, and were significantly higher than that under dark. The germination rate was fastest under red light, and lowest under blue lizht. There were no significant differences in germination percentages among the different light conditions for S. alpine seeds. The germination percentages under all light conditions were more than 93%. But S. alpine seeds germinated fastest under red light and dark, while lowest under blue light. The results indicated that cold storage, as a dormancy breaking treatment, had different effects on seed germination for diversified species ; however, as a trigger factor of germination, red light had consistent effects on seed germinat