中文摘要：

目的：探讨视网膜色素变性模型rd1小鼠视网膜退变中期各类视网膜节细胞（retinal ganglion cells,RGCs）功能的变化情况。方法：运用多电极阵列（multi-electrode arrays,MEA）记录方法,记录视网膜退变中期（出生后20 d,P20）的rd1小鼠或正常对照小鼠视网膜中多个节细胞动作电位的发放,并比较自发发放和光反应特征等指标,评价幸存的节细胞功能变化。另外,采用免疫组化染色方法验证视网膜感光细胞的退化情况。结果：免疫组化的结果表明rd1小鼠视网膜感光层的厚度显著低于正常小鼠。根据节细胞光反应特性的不同,可以将其分成6类：ON sustained、ON transient、ON-OFF sustained、ON-OFF transient、OFF sustained和OFF transient RGCs,但OFF sustained RGCs所占比重极小（1.0%~3.1%）。rd1小鼠视网膜中保持光反应的节细胞比例显著低于正常小鼠。rd1小鼠节细胞的自发发放显著高于正常小鼠,而不同类型的节细胞变化情况有所不同。rd1小鼠视网膜各类节细胞的光反应强度及光敏感度均显著低于正常小鼠。结论：在rd1小鼠退变的中期,视网膜感光层明显退变;rd1小鼠退变中期的视网膜节细胞发生明显的功能退变,而且不同类型的节细胞变化情况有所不同。

英文摘要：

AIM： To investigate how the function of retinal ganglion cells（ RGCs） change in the midterm of retinal pigmentosa（ RP） in rd1 mice（ a transgenic animal model of RP）. METHODS： The action potentials from multiple RGCs in rd1 mice at postnatal 20 d（ P20） or normal C57 mice（ control） were simultaneously recorded by multi-electrode array recording. The functional changes of surviving ganglion cells were evaluated by comparing spontaneous and light-evoked activities of RGCs between rd1 and control mice. The extent of photoreceptor degeneration was verified by immunohistochemical staining. RESULTS： Immunohistochemistry results showed the thickness of the retinal photoreceptor layer of rd1 mice was significantly lower than that in normal mice at P20. According to the light response properties,we classified ganglion cells into 6 subgroups： ON sustained,ON transient,ON-OFF sustained,ON-OFF transient,OFF sustained and OFF transient RGCs,with a very tiny percentage of OFF sustained RGCs（ 1. 0% ~ 3. 1%）. The percentage of RGCs remaining light responsive in rd1 mice was significantly lower than that in C57 mice. The average spontaneous spiking rate for rd1 RGCs was overall significantly increased compared to that in C57 cells,whereas different RGC types had different changes. The light-induced responses and light sensitivities of all types of RGCs in rd1 mice were both significantly lower than those in C57 mice. CONCLUSION： The photoreceptors of rd1 mice are severely degenerated in the midterm of retinal degeneration. The functions of RGCs in rd1 mice in the midterm of degeneration decay obviously,with variance in different RGCs types.