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中文摘要：
采用生化分析手段对菊黄东方鲀的未受精卵、胚胎（出膜前,受精后80~85 h）、初孵仔鱼（0日龄）、开口前仔鱼（3日龄）的脂肪酸组成和含量进行了检测和分析。结果显示,菊黄东方鲀卵和鱼苗的水分含量随着个体发育出现升高趋势,而鱼苗总脂含量随着个体发育显著下降。各发育阶段的干样中检出8种饱和脂肪酸（SFA）、7种单不饱和脂肪酸（MUFA）和12种多不饱和脂肪酸（PUFA）。菊黄东方鲀未受精卵脂肪酸组成与其亲本卵巢非常相似;胚胎期的脂肪酸利用率高低顺序为SFA（15.08%）、MUFA（14.46%）、n6PUFA（10.20%）和n3PUFA（0.19%）,以C16：0、C16：1、C18：1n9c和C18：2n6c为主要能量来源,n3PUFA被优先保存,特别是DHA得到完全保留;孵化后,在内源性营养阶段,仔鱼对n3PUFA利用迅速上升,仔鱼开口前的脂肪酸利用率高低顺序与胚胎期正好相反：n3PUFA（19.60%）、n6PUFA（16.98%）、SFA（13.50%）和MUFA（13.01%）,以C18：2n6c、C22：5n3（DPA）、C20：5n3（EPA）、C22：6n3（DHA）、C18：1n9c和C16：0为主要能量来源,其中仔鱼对DHA实际利用量为最高（14.44 mg/g）,仔鱼在开口前期n3PUFA（特别是DHA）被大量利用消耗。研究表明,菊黄东方鲀仔鱼发育需要消耗大量的n3PUFA（特别是DHA和EPA）,并建议在菊黄东方鲀苗种培育开口阶段及时增加富含EPA和DHA饵料的投喂量,如海水的轮虫、藻类强化过的卤虫幼体,缓解苗种开口阶段成活率低下的问题。
英文摘要：
In order to understand the value of changes in fatty acid composition during early developmental stage of tawny puffer Takifugu flavidus, the fatty acid composition and content of tawny puffer in different developmental stages（unfertilized eggs, embryos 80–85 h after fertilization, newly hatched larvae 0-day-age, and before feeding larvae 3-day-age） were detected and analyzed by biochemical analysis methods. The results show that the moisture of egg and fry increased significantly with ontogenesis; the total lipid contents of fry decrease significantly with ontogenesis. 8 saturated fatty acids（SFA）, 7 mono-unsaturated fatty acids（MUFA）, and 12 polyunsaturated fatty acids（PUFA） were found in the dry samples at different developmental stages. There was very similar tendency in fatty acid composition between unfertilized egg and its parent ovaries. At the embryonic period, the order of utilization rate of fatty acid was SFA（15.08%）, MUFA（14.46%）, n6PUFA（10.20%）, and n3PUFA（0.19%）, and C16：0, C16：1, C18：1n9c, and C18：2n6c were main sources of energy metabolism, and n3PUFA was preserved by priority, especially DHA, which was fully retained. After hatched, at the endogenous feeding stage, utilization rate of n3PUFA rose rapidly, followed by n3PUFA（19.60%）, n6PUFA（16.98%）, SFA（13.50%）, and MUFA（13.01%）. This was reverse to the embryo; C18：2n6c, C22：5n3（DPA）, C20：5n3（EPA）,C22：6n3（DHA）, C18：1n9c, and C16：0 were main sources of energy metabolism, and the highest practical utilized quantity was DHA（14.44 mg/g）, n3PUFA（especially DHA） was largely consumed before the larvae feeding.Therefore, larval development of tawny puffer needs to consume large amounts of n3PUFA（especially DHA and EPA）. In larval breeding of tawny puffer, in order to improve the survival rate of larvae, enhancement of bait enriched with DHA and EPA（e.g., seawater rotifera, and Artemia sp. nauplii enriched with algae） was suggested.
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