中文摘要：

目的：汉防己甲素和顺铂联合作用乳腺癌细胞，来提高癌细胞对顺铂的敏感性。方法：运用原子力显微镜对乳腺癌细胞表面的超微结构进行表征；MTT法和流式细胞术检测细胞的生长抑制率和细胞周期变化。结果：顺铂和汉防己甲素分别作用乳腺癌细胞48hIC50值为26．33μmol／1和5．6μmoL／1；联合用药组的IC50值为汉防己甲素2．5μmoL／L和顺铂13．32μmoL／l，在低浓度的联合用药作用乳腺癌细胞24h细胞膜表面结构被破坏，产生孔洞，作用48h被严重破坏使细胞周期在S比例增加为51．7％±0．30％。结论：提高了癌细胞对抗癌药物的敏感性，联合用药通过改变了细胞膜的结构对癌细胞进行有效杀伤，抑制肿瘤细胞生长。

英文摘要：

Objective： Improved the sensitivity of tumors to chemotherapeutic drugs was the key to cancer treatment. Method： MTr assay, Atomic force microscope and Flow Cytometer were used to study tetrandrine combined with cisplatin on human breast, cancer cell in vitro. Result： Inhibition of human breast cancer cell was treated with tetrandrine at different concentrations 48 hours. The concentration of tetrandfine at point of cell IC50 was 5.6μmol/1. When treated with cisplatin, the concentration was 26, 33μmol/1. When treated with both of them, the concentration of tetrandrine was 2.5 μmol/L, the other one was 13.32 μ moL/]. The surface of cell was damaged when treat with tetrandrine in low concentration at 24b and 48h. The S phase cell cycle percentage was improved to 51.7% ±0.30%. Conchtsion：lmproved the sensitivity of tumors to chemotherapeutic drugs treatment, inhibit tumor growth.