中文摘要：

以花后3，7，11周的“赤霞珠”（VitisviniferaL．CabemetSauvignon）葡萄果实为试材，应用荧光实时定量PCR技术，研究持续低温处理和变温处理对莽草酸途径人口酶3-脱氧-D-阿拉伯糖型-庚酮糖酸-7-磷酸合成酶（DAHPS）基因（VvDAHPS-1、VDAHPS-2和VvDAHPS-3）和后分支酸途径2个关键酶基因邻氨基苯甲酸合成酶（AS）以及分支酸变位酶（CM）基因（VvAS、VvCM-1和VvCM-2）表达的影响。结果表明，持续低温和变温处理对这些基因表达的影响有发育阶段的依赖性，2种温度处理均可显著地提高花后3周果实中3个VvDAHPS同源基因和VvCM-1的表达丰度，且VvDAHPS-1和VDAHPS-2受诱导的效应明显强于VvDAHPS-3和VvCM-1，随着果实发育，这些促进效应均明显减弱；2种温度处理对VvCM-2和VvAS表达的影响较小；这些基因对温度的响应也呈现处理时间的依赖性。笔者认为，一定低温有助于促进发育早期的葡萄果实中的碳源流向莽草酸途径合成更多的次生代谢物。

英文摘要：

In this report, grape （ Vitisvinifera L. full bloom were used as materials, real time PCR cv. ＇ Cabernet Sauvignon＇ ） berries at 3, 7 and 11 weeks after were performed, and the effects of treatments with constant low temperature and variable low temperature, respectively, on the expression of the genes encoding 3-Deoxy-D-ara- bino-heptulosonate-7-phosphate synthase （ DAHPS）, an entry enzyme of shikimate pathway, and anthranilic acid synthase （AS） and chorismatemutase （CM）, key enzymes of post-chorismate pathway were investigated. The results showed that the effects were developmental stage dependent, the treatments with constant and varia- ble low temperatures, both significantly increased the transcript abundance of three VvDAHPS isogenes and VvCM-1 in 3-week berries; the activation extent of expression of VvDAHPS-1 and VvDAHPS-2 was much higher than that of VvDAHPS-3 and VvCM-1, which were greatly reduced accompanying with berry mature; the expres- sion of VvCM-2 and VvAS was slightly affected by two low temperature treatments. Additionally, the response of these tested genes to low-temperature treatments was time dependent. These data suggested that a certain low- temperature will stimulate carbon flow in young grape berries to shikimate pathway, leading to the production more secondary metabolites.