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Screening compounds against HCV based on MAVS/IFN-β pathway in a replicon mode
  • ISSN号：1007-9327
  • 期刊名称：《世界胃肠病学杂志：英文版》
  • 时间：0
  • 分类：R512.63[医药卫生—临床医学;医药卫生—内科学]
  • 相关基金：Supported by The Natural Science Foundation of China,No.30600330,No.30671842,No.30672488,No.30700475,No.30771919and No.30700757;; the National High Technology Research and Development Program of China,No.2008AA02Z132;; Beijing Municipal Natural Science Foundation,No.5082016;; Mega-projects of Science Research for the 11th Five-Year Plan,No.2009ZX10004-4001
中文摘要：

AIM:To develop a sensitive assay for screening compounds against hepatitis C virus (HCV).METHODS:The proteolytic cleavage of NS3/4A on enhanced yellow fluorescent protein (eYFP)-mitochondrial antiviral signaling protein (MAVS) was examined by reporter enzyme secreted placental alkaline phosphatase (SEAP),which enabled us to perform ongoing monitoring of anti-HCV drugs through repeated chemiluminescence.Subcellular localization of eYFP-MAVS was assessed by fluorescence microscopy.Cellular localization and pr...

英文摘要：

AIM:To develop a sensitive assay for screening compounds against hepatitis C virus (HCV).METHODS:The proteolytic cleavage of NS3/4A on enhanced yellow fluorescent protein (eYFP)-mitochondrial antiviral signaling protein (MAVS) was examined by reporter enzyme secreted placental alkaline phosphatase (SEAP),which enabled us to perform ongoing monitoring of anti-HCV drugs through repeated chemiluminescence.Subcellular localization of eYFP-MAVS was assessed by fluorescence microscopy.Cellular localization and pr...

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期刊信息
  • 《世界胃肠病学杂志：英文版》
  • 主管单位:
  • 主办单位:百世登出版集团有限公司
  • 主编：
  • 地址：太原双塔西街77号
  • 邮编：100023
  • 邮箱：
  • 电话：0351-4078656
  • 国际标准刊号：ISSN：1007-9327
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