中文摘要：

建立了高效阴离子交换色谱一积分脉冲安培法（HPAEC-IPAD）直接检测烟叶和烟草料液中氨基酸的方法。利用离线除糖的方法，去除烟叶和烟草料液中大量干扰糖类，包括葡萄糖、果糖和蔗糖及麦芽低聚糖等。采用AminoPac PA10阴离子交换柱，以NaOH和NaAc的强碱性溶液为淋洗液，采用梯度洗脱，流速为0．25mL／min；积分脉冲安培法对氨基酸进行检测，回收率可达76％～105％。此方法可以有效的解决烟叶、烟草料液等含糖量高的样品中糖类化合物的干扰，对氨基酸实现灵敏、准确的定量分析。

英文摘要：

A method was established for the direct determination of amino acids in tobacco and tobacco feed liquid by high performance anion exchange chromatography with integrated pulsed amperometric detection （HPAEC-IPAD）. Using an off-line method, high concentrations of carbohydrates which can significantly interfere with amino acids determination were eliminated, including glucose, fructose, sucrose, oligosaccharides, and so on. The separation was carried out on an AminoPac PA10 anion-exchange column with NaOH/ NaAc gradient eluent at a flow rate of 0.25 mL/min. The determination was perfomed with integrated pulsed amperometric detector. The recoveries were in the range of 76% -105%. The method can be an efficient tool for the interfere elimination of high concentration of carbohydrates in samples such as tobacco and tobacco feed liquid, and accurate determination of amino acids.