中文摘要：

目的研究骨组织工程支架材料掺锶聚磷酸钙（strontium-doped calcium polyphosphate，SCPP）的降解产物（degradation products，DPs）对血管形成密切相关的内皮细胞迁移、增殖和肌动蛋白微丝（F-actin）重组的影响，探索其对骨组织工程血管化的作用。方法在聚磷酸钙（calcium polyphosphate，CPP）制备过程中掺锶制备SCPP多孔支架材料。生理盐水为降解介质，等离子体发射光谱检测降解液中DPs及浓度。用降解液处理脐静脉内皮细胞株ECV-304，噻唑蓝法和Millicell小室法分别检测细胞增殖和迁移。标记F-actin，荧光显微镜下观察。结果与CPP和生理盐水组比较。结果掺锶前后，孔隙率分别为（65±5）％和（64±5）％。SCPP各天降解液中Sr浓度为1．222～1．808mg／L，约为CPP组的20～40倍，但Ca和P较低。SCPP组细胞增殖和迁移数显著高于CPP组和生理盐水，细胞中F-actin重组形成应力纤维数量明显增多。结论SCPP的降解液能明显促进ECV-304的增殖。Sr是影响增殖的关键因素，SCPP降解液还能促进ECV-304的迁移，引起F-actin重组，形成与细胞迁移密切相关的应力纤维可能是促进迁移的原因之一。SCPP用作骨组织工程支架可能促进血管形成。

英文摘要：

Objective To investigate the effects of degradation proc/ucts（DPs） of strontium-doped calcium polyphosphate（SCPP） on migration, proliferation and F-actin reorganization of vascular endothelial cells, the crucial steps in angiogenesis, and to explore the potential of SCPP to promote angiogenesis in bone tissue engineering（BTE）. Methods Porous SCPP was prepared by introducing strontium（Sr） into calcium polyphosphate（CPP） with SrCO3 as Sr resource, Degradation fluid （DF） was prepared by immersing scaffolds in physiological saline under aseptic condition. DPs in DF was measured by inductively coupled plasma atomic emission spectrometry. ECV-304, a human umbilical vein endothelial cell line, was used and cultured with DF. Cell proliferation and migration were detected by MTT assay and Millicell chamber method respectively. F-actin was labled and examined using fluorescence microscope. Results of SCPP DF were compared with those of CPP and control （physiological saline）. Results The results showed that porosity ratio of SCPP scaffold is （64±5） %, which is similar with that of CPP（65±5） %. The concentration of Sr in SCPP DF is 1.222 ～ 1. 808 mg/L, which is about 20～40 times as high as that in CPP DF. Cell proliferation was significantly up regulated by DF of SCPP as compared with those of CPP and control. The F-actin and stress fibers of the migrated cells in SCPP group increased obviously. Conclusion Introduction of Sr into CPP don＇ t change the porosity ratio of scaffolds. DF of SCPP significantly promote proliferation and migration of vascular endothelial cells, and play important role in angiogenesis, Sr is proved to be the key factor for promoting cell proliferation. DF of SCPP induces F-actin polymerization and reorganization into stress fibers, which might be one of the reasons for improved cell migration. SCPP might be used in BTE for angiogenesis promotion.