中文摘要：

将前期从铊污染区筛选得到的9株高耐受性真菌菌株用于微生物对铊的富集和亚细胞分布实验，采用酶解和差速离心法分离各亚细胞组分，并检测其中铊的质量分数．结果表明，生物富集量随着铊处理浓度上升而降低，其影响趋势与对生物量的影响趋势基本一致，最高可达7189mg／kg，最大富集系数为7．19．在亚细胞水平上，铊的富集优先顺序为：细胞质一细胞壁一细胞器．亚细胞水平的区隔化作用是真菌对铊的主要耐受机制，细胞质是赋存铊的主要场所（53．83％～79．45％）．

英文摘要：

This paper focused on the sub-cellular distribution of thallium in several different fungus strains. The fungus strains were isolated from the Lanmuchang thallium-mineralised area in southwest Guizhou Province, and were used in the bio-concentration experiments. The sub-cellular supernatants including cytoplasmic, cellwall and cell organelles were seperated by the differential centrifuge after treated with snailase. Results show that the bio-concentration rate of thallium in the nine strains decreases with increasing thallium contents in the culture, the maximum at 7 189 mg/kg and the maximum bio- concentration factor （BCF） at 7. 19. In the sub-cellular supernatants detected by ICP-OES, the majority of thallium （up to 79.45% ） was found in cytoplasmic organelles, and the concentrations of thallium in the order of priority are as follows： eytoplasmic-cellwall-cell organelles. Cytoplasmic supernatant is apparently a major storage site for thallium, which is a possible detoxification of thallium by the fungus.