欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
原子力显微镜是观察DNA与内切酶相互作用比较有效的研究技术。实验中，首先把不同种类的内切酶与DNA在含有钙离子的缓冲液中培育30min，再通过原子力成像观察其相互作用后的复合物形貌。结果表明，二聚体内切酶EcoRV会结合到入．DNA的单个识别位点，最终形成珠串状结构，而且对DNA有弯曲作用：另一类型四聚体内切酶BspMI除了可以结合入-DNA的单个位点外，还可以结合2个识别位点而形成圆环状结构。此结果可以直观地观察到两种内切酶与DNA的作用形态，同时对DNA的弯曲与成环等结构给出直接证据。最后给出两种内切酶与DNA相互作用的模型图。
英文摘要：
Atomic force microscopy is effective research technology to observe the interaction between DNA and protein. First of all, in the experiment, the different kinds of enzyme and DNA were mixed in the buffer containing calcium ion, and were incubated for 30 minutes; Then the complex morphologies were observed by atomic force microscope. The results showed that dimer enzyme EcoRV may bind λ-DNA on single recognition sites to form the bead string structures and had bending action. Another tetramer enzyme BspMI not only combined single recognition of DNA, but also could combine the two recognition sites and form ring structure. The results could observe the interaction morphologies of two kind of enzyme and DNA directly, and gave direct evidence of DNA bending and ring structure. Finally, we gave the interaction model of two kinds of enzyme and DNA.
同期刊论文项目
同项目期刊论文
Morphology characterization and single-molecule study of DNA dodecyltrimethylammonium bromide comple
Mechanism of the hairpin folding transformation of thymine-cytosine-rich oligonucleotides induced by
Processes of DNA condensation induced by multivalent cations:Approximate annealing experiments and m
Theoretical investigation of the proton transfer mechanism in guanine-cytosine and adenine-thymine b
Canonical and micro-canonical analysis of folding of trpzip2: An all-atom replica exchange Monte Car
The conformational flexibility of nucleic acid bases paired in gas phase: A Car-Parrinello molecular
Kinetics and mechanism of G-quadruplex formation and conformational switch in a G-quadruplex of PS2.
Small organic molecules detection based on aptamer-modified gold nanoparticles-enhanced quartz cryst
Capability of DNA-fueled molecular machine in tuning association rate of DNA-functionalized gold nan
期刊信息
位置：