中文摘要：

目的：研究二苯烷基氨类衍生物氟桂利嗪（Flu）对小鼠生精细胞T型钙电流（ICaT）的影响。方法：采用急性机械分离的小鼠生精细胞，应用膜片钳全细胞记录技术，观察Flu对ICaT的影响。结果：钳制电位为-90mV、刺激电压为-30mV时，Flu（0．1、1、10、100μmol／L）可抑制小鼠生精细胞Ca^2＋电流，抑制率分别为（23．34±2．76）％、（46．04±3．52）％、（62．52±3．70）％、（73．52±3．12）％（P〈0．05）；Flu对T型Ca^2＋通道的半数最大抑制浓度为0．29μmol／L。结论：Flu对生精细胞T型Ca^2＋通道有阻断作用，呈浓度依赖性；Ca，3．2是生精细胞T型Ca^2＋电流的主要贡献者。

英文摘要：

Objective： To investigate the effects of Flunarizine （Flu） on T-type calcium currents （ ICaT ） in spermatogenic ceils. Methods： Ca^2＋ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed. Results： Flu of 0.1,1,10,100 μmoL/L inhibited ICaT in mouse spermatogenic cells significant- ly with the K50 value of 0.289 μmoL/L（ P 〈 0.05 ）. With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were （23.34±2.76）%, （46.04±3.52）%, （62.52±3.70）% and （73.52 ±3.12）%, respectively. Conclusion ： Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca,3.2 is the main contributor to T-type Ca^2＋ currents in spermatogenic cells.