中文摘要：

目的：研究甲基化酶抑制剂5-杂氮脱氧胞苷（5-aza-2‘-deoxycytidine，ADC）对人绒毛膜上皮癌细胞株JEG-3细胞的生物学作用，观察JEG-3细胞H19表达变化与滋养细胞增殖、分化和侵袭等行为的相关性，从表观遗传学角度探讨滋养细胞侵袭行为的调控。方法：应用MTT法检测不同浓度5-杂氮脱氧胞苷在不同时相下对JEG-3细胞增殖的影响。体外小室迁移实验观察5-杂氮脱氧胞苷对JEG-3细胞迁移的影响。荧光定量RT—PCR检测5-杂氮脱氧胞苷作用下JEG-3细胞H19mRNA表达的变化。结果：5-杂氮脱氧胞苷对JEG-3细胞增殖起抑制作用，在一定范围内与时间和剂量呈正相关。经100μmoL／L5-杂氮脱氧胞苷作用24h后的JEG-3细胞迁移能力明显受限，迁移细胞数较对照组差异显著（P〈0.01）。经5-杂氮脱氧胞苷作用后JEG-3细胞中H19mRNA表达增高，在一定范围内与时间和剂量呈正相关。结论：甲基化酶抑制剂5-杂氮脱氧胞苷可致H19 mRNA过量表达，并进一步抑制滋养细胞的增殖和迁移能力。H19印迹基因表达上调与滋养细胞增殖抑制和迁移能力降低有关。

英文摘要：

Objective： To explore the transcription regulation of 5-aza-2＇-deoxycytidine （ADC） on H19 gene and its effect on JEG-3 cell line growth and migration. Methods： MTT method was used to detect the proliferation of JEG-3 cells after treated with altered dosage of 5- aza-2＇-deoxycytidine. JEG-3 cells migratory function were determined by the ability of cells to migrate through 8μm pores of polycarbonate membranes fitted to the bottom of transwell migration chambers. H19 mRNA level were detected by relative quantitative real-time PCR （rqRTPCR）. Results：After 5-aza-2＇-deoxycytidine treatment, the profileration and the migration ability of JEG-3 cells was depressed, while the level of H19 mRNA expression was increased significantly. Conclusion：5-aza-2＇-deoxycytidine can effectively upregulate the expression of H19 mRNA. Over-expression of H19 gene is asscioated with the profileration and the migration ability of JEG-3 cell line.