中文摘要：

目的：建立延胡索药材的RP—HPLC指纹图谱，研究不同产地延胡索药材的质量差异，为延胡索药材制定质量标准提供参考。方法：应用Phenomenex LunaC18（250mm×4．6mm，5μm）色谱柱，柱温30℃，以甲醇一磷酸盐缓冲溶液为流动相梯度洗脱，流速1．0mL·min^-1，检测波长289nm；采用“中药色谱指纹图谱相似度评价2004A版”软件比较不同产地炮制与未炮制延胡索药材指纹图谱的相似度，利用对照药材指纹图谱对21批样品进行聚类分析。结果：不同产地炮制和未炮制延胡索药材指纹图谱稍有不同，分别建立共有模式，相似度较好；聚类分析结果表明个别药材质量较差。结论：炮制与否对延胡索药材的化学组成有所影响，但各地延胡索药材质量差异不大，采用RP—HPLC方法分别建立炮制和未炮制延胡索药材的指纹图谱，方法重现性好，可用于延胡索的质量评价和品种鉴别。

英文摘要：

Objective ： To establish HPLC - fingerprint of Rhizoma Corydalis and to compare the quality of Rhizoma Corydalis from different habitats. Methods：The gradient elution mode was applied in chromatographic separation, and ＂Chromatographic Fingerprint Similarity Evaluation＂ software was used to analyse data and evaluate HPLC - fingerprint established. Hierarchical cluster analysis was made with comparison of fingerprint for 21 samples of Rhizoma Corydalis. Results： There was a difference in HPLC - fingerprints between the herbs with and without pre - preparation,but the samples from different habitats were of high similarity, only a few samples had slightly differ- ence. Conclusions：The RP - HPLC fingerprint method is repeatable, feasible in analysis of Rhizoma Corydalis and can be used in quality assessment of Rhizoma Corydalis. The chemical components of Rhizoma Corydalis are dependent on with and without pre- preparation process and hardly on habitats.