中文摘要：

设计并制作了一种集成有8个重复6×6细胞培养单元的阵列微流控细胞芯片,以实现细胞培养和系列植物组分的细胞抗氧化活性（Cellular antioxidant activity,CAA）分析。芯片主要包含聚二甲基硅烷盖片、288个圆形培养腔体,48个独立平行通道的玻璃基底层,一次可完成8个样本的6个浓度筛选,并可在酶标仪上实现测试。槲皮素、芦丁和山奈酚等植物组分与芯片上培养的细胞作用24 h,细胞存活率大于90%。以芯片上培养的人肝癌细胞HepG2为细胞载体,以2＇,7＇-二氯荧光素-乙酰乙酸酯（2＇,7＇-Dichlorofluorescin diacetate,DCFH-DA）为荧光探针,采用2,2＇-偶氮二异丁基脒二盐酸盐（2,2＇-azobis（2-amidinopropane）dihydrochloride,ABAP）为细胞内活性氧（Reactive oxygen species,ROS）引发剂,测得槲皮素、芦丁、山奈酚等植物组分的CAA unit分别为71.42±0.19、74.31±0.36和69.92±0.09（±s,n=3）,IC_（50）分别为（7.20±0.06）μmol/L,（52.06±0.14）μmol/L,（32.55±0.03）μmol/L（±s,n=3）。

英文摘要：

An integrated microfluidic chip with arrayed micro channels that consisted of eight repeat arrayed 6× 6 cell culture chamber was designed and fabricated. The analytical microsystem combined with designed microchip,measuring device and environmental control unit was established for cell culture and parallel cellular antioxidant activity（ CAA） analysis of plant antioxidants. The microfluidic chip included a polydimethylsiloxane（ PDMS） cover and glass substrate that consisted of two hundreds and eighty-eight round cell culture microchambers and forty-eight independent parallel array channels. Eight groups of different samples with six different concentrations could be investigated simultaneously with multimode reader in one test. Hep G2 cells were successfully cultured on the microchip. Moreover,the viability percentage of the Hep G2 cells exposed to these plant antioxidants solutions at different concentrations for 24 h was higher than90%. With 2＇,7＇-Dichlorofluorescin diacetate（ DCFH-DA） as a fluorescence probe,2,2＇-azobis（ 2-amidinopropane） dihydrochloride（ ABAP） as the initiator of intracellular reactive oxygen species（ ROS）,we tested the inhibitory effect of several plant antioxidants such as quercetin,rutin and kaempferol on free radicals. The CAA units were calculated by the data measured from cellular morphology and fluorescence intensity over time. It was shown that the CAA units of quercetin,rutin and kaempferol were 71. 42 ± 0. 19,74. 31 ± 0. 36 and 69. 92 ± 0. 09（ x± s,n = 3）,while the calculated IC_（50） were（ 7. 20 ± 0. 06） μmol / L,（ 52. 06 ± 0. 14） μmol/L and（ 32. 55 ± 0. 03） μmol/L（ x± s,n = 3）,respectively.