中文摘要：

文中以家蚕翅原基为材料,通过制做离体石蜡切片的方法观察了翅原基从五龄幼虫第3天至蛹期0天的形态变化,并通过注射外源蜕皮激素活性物质20E和保幼激素类似物Methoprene探究了昆虫激素对家蚕翅原基生长分化的影响.结果显示,翅原基在幼虫阶段发育缓慢,从五龄幼虫第6天起生长分化逐渐加快,且翅原基的形态发生了显著变化,原本附着于翅原基腔口处且呈团状的造血器官逐渐分散至消失,而由气管组成的翅脉逐渐形成.外源激素处理结果显示,2μg的20E可促进翅原基的生长分化,而2μg的Methoprene抑制了翅原基的生长分化.上述结果说明蜕皮激素和保幼激素共同调控了翅原基的生长分化,并最终实现了翅原基的变态发育.

英文摘要：

The morphology of wing discs from day 3 of 5th larval instar to day 0 of pupa was observed by separated wing disc and the paraffin sections.To determine whether the growth and differentiation of wing discs were regulated by insect hormone,ecdysone 20E and juvenile hormone analogue Methoprene were injected into the silkworm at day 3 of 5th larval instar separately.The results showed that the wing discs grew slowly at larval stage but grew quickly from day 6 of 5th larval instar.What＆#39;s more, the morphology and structure of wing discs changed significantly.The hematopoietic organ degraded gradually and the veins formed finally.The development and differentiation of the wing discs were positively regulated by the certain dose of 20E and negatively regulated by Methoprene.The above results indicated that ecdysone and juvenile hormone regulated the growth and differentiation of wing discs, resulted in the metamorphosis of wing discs.