对刺五加叶总黄酮提取工艺进行了研究,同时利用1,1-二苯基-2-苦基苯肼自由基(DPPH)法和羟基自由基(OH)法对刺五加叶总黄酮提取物进行了的抗氧化评价。结果表明:超声提取法对刺五加叶总黄酮提取效果最佳。超声提取正交试验结果表明,刺五加叶总黄酮的最佳提取工艺为:以50%乙醇溶液提取,提取时间为1.5h,提取次数为2次,溶媒倍量为20倍,提取率为5.192%。抗氧化活性结果表明,刺五加叶总黄酮对DPPH和.OH均有良好的清除作用,且随着其浓度的增加,清除能力增强。
Objective:The extraction methods of Flavonoids have been studied,and the extraction process has been optimized.And then,the antioxidant activity of total flavonoids extract against DPPH and hydroxyl radical has been evaluated.The experimental.Methods:Results:show that the ultrasonic extraction method is best by compared with the reflux method and accelerated solvent extraction and extraction,and the optimization extraction conditions are as follows:ethanol concentration is 50%,extraction time is 1.5h,extraction times is 2 times,solvent times is about 20 times.The extraction rate is 5.192%.The removal capacity of the extract on DPPH and OH radical is best.With the.Conclusion:increasing,the removal capacity is increasing.