中文摘要：

以氧化石墨烯（GO）为固定酶载体，在水溶液中通过静电吸附及氢键作用实现溶菌酶（Ly）的固定化得到GO-Ly。并将已固定化酶的GO为添加剂，以聚醚砜为膜材料，采用相转化法制备杂化超滤膜。考察了添加剂含量对膜形态、亲水性、分离性能、力学性能及抗菌性能的影响。结果表明，GO-Ly的加入使杂化膜的亲水性及纯水通量得到明显提高，同时拉伸强度也得到一定改善；尤其当GO-Ly添加量为1.5%（质量分数）时，膜的纯水通量达到318 L/（m2？h），并且对聚乙烯醇（PVA 30000~70000）的截留率维持在99%以上，对大肠杆菌的抑菌率可达68%。

英文摘要：

Graphene oxide （GO） provides an ideal substrate for lysozyme immobilization due to a large specific surface area and abundant functional groups. The lysozyme immobilization on the GO sheets could take place readily by electrostatic interaction of GO lysozyme （GO Ly）. The immobilized lysozyme of GO used as the addictive was blended with polyethersulfone （PES） to prepare hybrid ultrafiltration membrane via phase inversion. The effect of GO Ly con tent on the morphology,hydrophilicity,separation,mechanical and antibacterial properties of hybrid membranes was investigated. The results showed that the surface hydrophilicity and wa ter flux were significantly improved with the increase of GO Ly content. Meanwhile,the me chanical strength of hybrid membrane was also increased. Especially when GO Ly content was 1.5 %,the water flux reached up to 318 L/（m2？h）. The rejection of polyvinyl alcohol （PVA 30 000~70 000） was 99 % and the antibacterial rate of the hybrid membrane against E. coli could achieve 68%.