中文摘要：

【目的】建立超高效液相色谱法同时测定13批不同产地大黄中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素等5种游离蒽醌的含量。【方法】采用WatersBEHC18（2．1mm×150mm，1．8μm），以乙腈（A）-0．1％甲酸水溶液（B）为流动相，梯度洗脱[0—3min，19％-＋25％A，3-10min，25％--100％A]，流速0．3mL／min，柱温30℃，检测波长254nm。【结果】大黄酸、大黄酚、大黄素、大黄素甲醚、芦荟大黄素分别在1．61～80．30，4．98～249.20，3．27-163．30，9．65～482．50，1．67～83．30μg／mL范围内呈现良好的线性关系，平均回收率分别为99．7％，100．4％，100．1％，98．9％，100．9％。相对标准偏差（RSD）分别为1．5％，0．6％，0．8％，0．4％，1．7％。【结论】该方法简便可行，重复性良好，可用于大黄药材的质量控制。

英文摘要：

[Objective] To develop a UPLC method for simultaneous determination of five anthraquinones （Rhein, Chrysophanol, Emodin, Physcion and Aloe-emodin） in Radix et Rhizom a Rhei from different habitats. [Methods] The analysis was performed on Waters BEH C18 column （2.1 mm×150 mm, 1.8 μm） with a gradient mobile phase of acetonitrile （A）-0.1% Formic acid solution （B）（0N3 min, 19%→25% A, 3-10 min, 25%→100%A） at flow rate of 0.3 mL/min. The column was maintain at 30℃ and the detection wavelength was set at 254 nm. [Results] The linear ranges were 1.61 -80.30 μg/mL for Rhein, 4.98 N249.20 μg/mL for Chrysophanol, 3.27-163.30 μg/mL for Emodin, 9.65 -482.50 μg/mL for Physcion and 1.67-83.30 μg/mL for Aloe-emodin. The average recoveries of the five compounds were 99.7%, 100.4%, 100.1%, 98.9%, 100.9% and RSD were 1.5%, 0.6%, 0.8%, 0.4%, 1.7% respectively. [Conclusion] The rapid and accurate UPLC method was established. This method can be used to assess the quality of Rhubarb.