中文摘要：

多能干细胞（pluripotent stem cells,PSCs）作为一类重要的细胞资源,成为目前国内外的研究热点,获得这类细胞在当今医学研究和生物技术的发展及应用中具有重要的意义.猪（Sus scrofa）由于其生理结构和代谢特征与人（Homo sapiens）较为相似,成为动物多能干细胞建系的重点研究对象.然而目前,猪多能干细胞建系工作仍存在很多难题.本文将从猪多能干细胞建系研究的进展和存在的问题入手,对照小鼠（Mus musculus）和人相关研究的成功经验,总结目前已经报道的猪多能干细胞的培养条件、调控因子、信号通路和基因表达特征,以期为建立猪胚胎干细胞和诱导多能干细胞系提供参考.

英文摘要：

Pluripotent stem cells （PSCs）,which are regarded as important cell resources,have become the research focus around the world in recent years.Acquisition and acquaintance of these cells are of significance in medical research and biotechnology application.Pig（Sus scrofa） is considered to be the most important domestic animal in PSCs establishment,because of its similarities in physiology and metabolic to human（Homo sapiens）.For now,however,pig PSCs establishment are still facing a lot of problems.Combined with the successful experiences of the PSCs research of mouse（Mus musculus） and human,we summarized cultural conditions,regulatory factors,signaling pathways,and gene expression profiling of those reported porcine PSCs.We provide useful information for the establishment of defined embryonic stem cells（ESCs） and iPSCs of pig.