利用傅里叶变换红外光谱法对59份来自中国6个不同产地的野生和栽培甘草原药材及其提取物进行了分析研究。通过比较甘草原药材及其提取物与甘草的指标成分甘草酸和甘草苷的一维及其二阶导数光谱,发现1100~1000cm^-1范围内的峰是甘草多糖类成分的特征峰;1318cm^-1是草酸钙的特征峰;1745,1386和1612,1512cm^-1分别是甘草酸和甘草苷的特征指纹峰;特征峰的强度可代表相应化学成分的含量。根据上述特征峰强弱对所有样品进行横纵向比较,结果显示:野生甘草特征峰强,相应指标成分含量高;栽培甘草特征峰弱,相应指标成分含量低;甘草药材质量与生长年限密切相关。
Fourier transform infrared spectroscopy with a spectra range of 4 000-400 cm^-1 was used to identify fifty nine samples of wild or cultivated Glycyrrhiza uralensis Fisch. from six different places around China. The 1D spectra and their second derivative spectra of herbal samples,the corresponding extract samples and two marker compounds (glycyrrhizic acid and liquiritin) were compared. The wide peak in 1 100-1 000 cm^-1 was used to identify the components of polysaccharide; the peak at 1 318 cm^-1 mainly belonged to calcium oxalate; the peaks at 1 745 and 1 386 cm^-1 were used to assess the content of glycyrrhizic acid; the peaks at 1 612 and 1 512 cm^-1 were the characteristic peaks of liquiritin. The intensity of characteristic peaks can represent the content of corresponding compound. The horizontal and vertical comparisons of all herbal samples showed that the characteristic peaks of wild samples were strong,and all wild samples had the similar quality; the characteristic peaks of cultivated samples were weaker than those of the wilds; the ages of glycyrrhiza had great effect on the quality of samples