以涂布法和富集培养法筛选得到降解菌株HQ—C-01,该菌株在48h时对50mg/L克百威、甲萘威、茚虫威和仲丁威的降解率分别可达95.2%、99.0%、85.O%和67.5%.确定菌株HQ—C-01的C、N、P源化合物为葡萄糖、蛋白胨和磷酸氢二钾,采用中心组分设计法确定了菌株HQ—C-01的培养基最适C、N、P源配比为葡萄糖32.10g/L、蛋白胨3.25g/L、磷酸氢二钾1.50g/L;在最佳培养基条件下,D590nm实测值为0.7865,与期望值0.8054接近,48h时对50mg/L克百威降解率为95.9%.
A high effective stain named HQ-C-01 carbofuran, carbaryl, indoxacarb and fenobucarb was screened, which the degrading rates for 50 mg/L after treatment in 48 h were 95.2%, 99. 0%, 85.0% and 67.5% respectively. It was found that the compounds for C, N and P were glucose, peptone and potassium phosphate dibasic. By using the central composite rotary design method, the results indicated that the optimum of C ,N and P were 32. 10 g/l. for glucose ,3.25 g/L for peptone and 1. 50 g/L for potassium phosphate dibasic respectively. The measured value of D590 was 0. 786 5 similarly to the expected value, and the degrading rate of 50 mg/L carbofuran was 95.9% after 48 h by the strain incubation in medium optimization.