中文摘要：

目的探讨程序性细胞死亡因子配体1（Pd-L1）在乙型肝炎不同病程期间肝组织中的表达，以阐明其在HBV感染慢性化发生发展中的作用。方法用免疫组织化学方法结合图像定量分析系统检测65例慢性HBV感染者和5例健康对照者肝组织内PD-l1的表达．并与肝组织炎症活动度分级、TBil、ALT、AST及HBVDNA进行相关分析。结果健康对照组肝组织PD-L1为（0．64±0．28）%，显著低于慢性乙型肝炎组，分别为：G0（3．07±0．93）％，G1（8．01±1．49）％，G2（11．60±2．60）％，03（18．41±2．21）％，G4（26．04±3．41）%，其表达量与肝脏炎症活动度分级、TBil、AI，T、AST呈正相关（r=0．917、0．787、0．483、0．628，P〈0．05），与HBVDNA载量呈负相关（r=0．620，P％0．05）。结论PD-L1在肝组织中表达上调可能参与了HBV感染慢性化的发病机制。

英文摘要：

Objective To investigate expressions of programmed cell death ligand 1 （PD-L1） in hepatic tissues at the different stages of hepatitis B virus （HBV） infection, and clarify its role in the mechanism of chronic hepatitis B virus infection. Methods The expressions of PD-L1 were detected by immunohistochemistry and computer image quantitative analysis in the hepatic tissues of 65 chronic HBV infected patients and 5 healthy controls. The correlations between PD-L1 expression and inflammatory grading in the hepatic tissues, total bilirubin （TBil）, alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, serum HBV DNA level were analyzed. Results The Pd-L1 expressions in hepatic tissues of HBV infection with GO -- G4 inflammatory grades were 3. 07 % ＋ 0.93%, 8.01%±1.49%, 11.60%±2.60%, 18.41%±2.21% and 26.04%±3.41%, respectively, which were all significantly stronger than that in controls （0.64 % ±0.28 % ）. PD-L1 expression was a positively correlated with inflammation grading of hepatitis tissues, TBil, ALT and AST level in serum （r=0. 917, 0. 787, 0. 483, 0. 628; all P〈0.05）, and negatively correlated with serum HBV DNA load （r= -0. 620, P%0.05）. Conclusion The upregulated PD-L1 expression may be probably involved in the chronicity of HBV infection.