中文摘要：

目的研究有机化学污染物对Vero细胞状态及质膜组分的影响，以阐明Vero细胞变形的分子机制，为建立一种基于Vero细胞变形的微量化学污染物生物毒性定量分析方法提供理论基础。方法选取了典型污染物2，4，6-三氯苯酚（2，4，6-trichlorophenol，TCP）为研究对象，采用流式细胞术分析了不同浓度（0、1、5、20、50mg/L）TCP对Vero细胞状态的影响。采用两相分配法提取了具有活力的细胞质膜，运用膜组分分析技术研究了不同浓度（0、25、50、75、100、125mg／L）TCP对Vero细胞状态和细胞膜结构、组分的影响。结果与对照（0mg／L）相比，1、5、20、50mg／L的TCP就可导致Vero细胞的变形。随着TCP染毒浓度的升高，活细胞数呈下降趋势，死细胞数呈升高趋势。污染物在导致细胞凋亡与坏死前，先致Vero细胞膜的损伤。随着TCP浓度的升高，变形Vero细胞质膜中蛋白和磷脂含量呈下降趋势，蛋白，磷脂比值呈升高趋势。结论TCP对Vero细胞质膜中蛋白和磷脂含量以及蛋白／磷脂比值的影响可能导致细胞膜刚性结构的变化，进而导致细胞形态异常。

英文摘要：

Objective To elucidate the molecular mechanism of cell deformation and to provide the theoretical guidance for quantitative analysis of toxicity of trace chemical contaminants based on the Veto cell deformation. Methods Vero cells state affected by typical organic pollutant TCP was analyzed with the method of flow cytometry. The structure and composition of cell plasma membrane affected by TCP at the exposure doses of 0,25,50,75,100 and 125 mg/L were analyzed with the method of two phase distribution membrane extraction and membrane fraction analysis. Results Compared with the control group, different concentrations （1,5,20,50 mg/L） of organic pollutants （TCP） had led to the significant deformation of cells. With the elevated concentration of TCP, the number of viable cells showed a decreasing trend and the dead cells showed an increasing trend. The organic pollutant induced the plasma damage before the cell proliferation and death. Further study showed that membrane protein and phospholipids contents accounted for the proportion of dry cell weight cultured with TCP （25,50,75,100,125 mg/L） were lower than those in normal cells, the ratio of protein to phospholipids of plasma membrane increased, with a dose dependent manner. Conclusion The changes of phospholipids to plasma membrane protein contents and ratio in cell membrane may cause the change of rigid structure and the deformation of cells, so this method is applicable to the quantitative analysis of toxicity of chemical contaminants to cells.